香港远志化学成分研究

香港远志(Polygala hongkongensis Hemsl.)系远志科(Polygalaceae)远志属植物，主要分布于江西、湖南、广东和四川等南方地区。民间用于活血、化痰，并具解毒作用。目前从远志属植物中分离得到的化学成分主要为皂苷、酮和糖酯类化合物。由于远志属植物化学成分的多样性和生物活性的显著性，作者首次对香港远志进行了化学成分的研究，以期寻找新化合物和活性成分。本文采用70%甲醇提取，减压回收溶剂得浓缩物，将浓缩物溶于水中，分别用醋酸乙酯和正丁醇萃取。取正丁醇萃取物，用大孔吸附树脂、硅胶、凝胶及半制备HPLC等色谱技术反复分离纯化化学成分，根据化合物的理化性质和UV， IR， HRESI-MS， ESI-MS， ¹H NMR， ¹³C NMR， HMQC， HMBC， H-H COSY， NOESY等光谱数据进行结构鉴定。从香港远志的正丁醇萃取部位分离得到6个化合物，分别鉴定为香港远志碱(polyhongkonggaline，1)，3,6′-二芥子酰基蔗糖酯(3,6′-di-O-sinapoyl-sucrose，2)，tenuifoliside A (3)，glomeratose D (4)，顺式丁香苷(cis-syringin，5)，丁香脂素-4′-O-β-D-单葡糖苷(syringaresinol-4′-O-β-D-monoglucoside，6)。其中化合物1为新化合物，2～6均为首次从该植物中分离得到。

The chemical constituents of Polygala hongkongensis Hemsl.

Polygala hongkongensis Polycalaceae is mostly distributed in southern China, such as Guangdong, Jiangxi, Fujian and Sichuan provinces. And its herbs is used as a remedy of heat-clearing and detoxicating, removing food retention, promoting blood flow and expelling phlegm to arrest coughing in the folk medicine. Previous phytochemical investigations on Polygala plants have reported that the main chemical constituents are sapaonins, xanthones and oligosaccharide esters. To the best of our knowledge, there is no chemical report on the Polygala hongkongensis Hemsl. yet. In order to search and make use of natural resources from Polygala and to find the bioactive compounds and new compounds, we carried out studies on chemical constituents of this plant. The herbs of P. hongkongensis were extracted with 70% MeOH. The extract was combined and evaporated in vacuum to residue, which was suspended in water and successively partitioned with EtOAc and n-BuOH. Part of the n-BuOH extract was isolated and purified by various column chromatographs such as a macroporous resin, silica gel, Sephadex LH-20 column and semipreparative HPLC. The structures of isolated and purified compounds were determined by spectral analysis such as UV, IR, HRESI-MS, ESI-MS, 1H NMR, 13C NMR, HMQC, HMBC, H-H COSY, NOESY and physico-chemical property. Six compounds were identified as polyhongkonggaline (1), 3, 6'-di-O-sinapoyl-sucrose (2), tenuifoliside A (3), glomeratose D (4), cis-syringin (5), syringaresinol-4'-O-beta-D-monoglucoside (6). Compounds 1 is new compound, and 2-6 were isolated from this plant for the first time. Farther studies on the chemical constituents and pharmacological activities of P. hongkongensis will be carried out.