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| 基于双基因分析的结肠癌标志基因选择 | |
| 引用本文： | 李建更,高志坤,严志,阮晓钢. 基于双基因分析的结肠癌标志基因选择[J]. 中国生物医学工程学报, 2009, 28(5) |
| 作者姓名： | 李建更 高志坤 严志 阮晓钢 |
| 作者单位： | 1. 北京工业大学人工智能与机器人研究所,北京,100124 2. 北京肿瘤分子生物学实验室,北京市肿瘤防治研究所,北京大学临床肿瘤学院,北京,100036 |
| 基金项目： | 国家自然科学基金重点项目，北京市教育委员会发展计划资助项目 |
| 摘 要： | 针对结肠癌标志基因选择问题,引入一种最高得分对(TSP)方法,处理一组包含40个肿瘤和22个正常样本的结肠癌微阵列数据,得到标志基因对(VIP,DARS)并构建双基因分类器.结果显示,该分类器对62例样本的分类准确率可达93.55%.进一步,利用荧光实时定量PCR在10个独立样本上检验所选基因对的分类效果,正确率为100%,表明该方法能有效地选择结肠癌标志基因,对临床诊断有积极的意义. |
| 关 键 词： | 标志基因 结肠癌 双基因分类器 荧光实时定量PCR(Q-RT-PCR) |
Marker Gene Selection of Colon Cancer Based on Two-gene Analysis |
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| LI Jian-Geng,GAO Zhi-Kun,YAN Zhi,RUAN Xiao-Gang. Marker Gene Selection of Colon Cancer Based on Two-gene Analysis[J]. Chinese Journal of Biomedical Engineering, 2009, 28(5) | |
| Authors: | LI Jian-Geng GAO Zhi-Kun YAN Zhi RUAN Xiao-Gang |
| Abstract: | For the selection of marker gene for colon cancer, a method based on top scoring pair (TSP) was introduced to process a microarray dataset including 40 tumor and 22 normal colon tissue samples. A pair of genes, VIP and DARS, was obtained as marker genes. According to a two-gene classifier constructed by the two genes, the 62 samples were distinguished with the accuracy of 93.55%. Furthermore, this pair of genes was validated with quantitative real-time PCR on an additional 10 independent samples, and gained the accuracy of 100%. The outcome showed that this method could select marker genes for colon cancer effectively and was promising in clinical diagnosis. |
| Keywords: | marker gene colon cancer two-gene classifier quantitative real-time PCR (Q-RT-PCR) |
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