Assessment of the transfer,persistence, prevalence and recovery of DNA traces from clothing: An inter-laboratory study on worn upper garments |
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| Affiliation: | 1. School of Life and Environmental Sciences, Deakin University, Geelong, Australia;2. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, Macleod, Australia;3. Swedish National Forensic Centre, Linköping, Sweden;4. Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden;5. Forensic Science Ireland, Garda HQ, Phoenix Park, Ireland;6. Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Amsterdam, the Netherlands;7. Division Biological Traces, Netherlands Forensic Institute, The Hague, the Netherlands;8. School of Molecular Sciences, La Trobe University, Bundoora, Australia;1. Division Biological Traces, Netherlands Forensic Institute, P.O. Box 24044, 2490 AA, The Hague, The Netherlands;2. Swedish National Forensic Centre, SE-581 94, Linköping, Sweden;3. Department of Physics, Chemistry and Biology (IFM), Linköping University, SE-581 83, Linköping, Sweden;4. Forensic Science Ireland, Garda HQ, Phoenix Park, Dublin 8, Ireland;5. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, P.O. Box 94248, 1090 GE Amsterdam, The Netherlands;6. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, 31 Forensic Drive, Macleod, Vic. 3085, Australia;7. School of Life and Environmental Sciences, Centre for Chemistry and Biotechnology, Deakin University, Locked Bag 20000, Geelong, Vic. 3220, Australia;1. UCL Centre for the Forensic Sciences, 35 Tavistock Square, London WC1H 9EZ, UK;2. UCL Division of Biosciences, Medical Sciences Building, Gower Street, London WC1E 6BT, UK;3. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, 31 Forensic Drive, Macleod 3085, Australia;4. UCL Department of Security and Crime Science, 35 Tavistock Square, London WC1H 9EZ, UK;1. Forensic Science South Australia, 21 Divett Place, Adelaide, SA 5000, Australia;2. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia;3. Faculty of Law, Criminal Justice and Public Administration, School of Criminal Justice, University of Lausanne, 1015 Lausanne-Dorigny, Switzerland;4. Fondation pour la Formation Continue UNIL-EPFL, University of Lausanne, 1015 Lausanne-Dorigny, Switzerland;1. Central Criminal Investigations Division, National Police of the Netherlands, The Netherlands;2. Crime Scene Support Team, Netherlands Forensic Institute, The Netherlands;3. Division of Biological Traces, Netherlands Forensic Institute, The Netherlands;4. School of Molecular Sciences, La Trobe University, Bundoora, Australia;5. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Centre, Australia;1. Division Biological Traces, Netherlands Forensic Institute, P.O. Box 24044 2490 AA, The Hague, the Netherlands;2. Swedish National Forensic Centre, SE-581 94, Linköping, Sweden;3. Department of Physics, Chemistry and Biology (IFM), Linköping University, SE-581 83, Linköping, Sweden;4. Forensic Science Ireland, Garda HQ, Phoenix Park, Dublin 8, Ireland;5. Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, P.O. Box 94248, 1090 GE, Amsterdam, the Netherlands;6. Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, 31 Forensic Drive, Macleod, Vic, 3085, Australia;7. School of Life and Environmental Sciences, Deakin University, Locked Bag 20000, Geelong, Vic, 3220, Australia;8. School of Molecular Sciences, La Trobe University, Bundoora, Vic, 3086, Australia |
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| Abstract: | ![]()
Among the various items recovered from crime scenes or persons involved in a crime event, clothing items are commonly encountered and submitted for forensic DNA sampling. Depending on the case circumstances and the activity-of-interest, sampling of the garment may concentrate on collecting DNA from the wearer, or from one or more offenders who have allegedly contacted the item and/or wearer. Relative to the targeted DNA, background DNA already residing on the item from previous contacts, or transferred during or after the crime event, may also be collected during sampling and observed in the resultant DNA profile. Given our limited understanding of how, and from where, background DNA is derived on clothing, research on the transfer, persistence, prevalence, and recovery (TPPR) of DNA traces from upper garments was conducted by four laboratories. Samples were collected from several areas of two garments, each worn on separate working or non-working days and individually owned by four individuals from each of the four laboratories, and processed from DNA extraction through to profiling. Questionnaires documented activities relating to the garment prior to and during wearing, and reference profiles were obtained from the wearer and their close associates identified in the questionnaire. Among the 448 profiles generated, variation in the DNA quantity, composition of the profiles, and inclusion/exclusion of the wearer and their close associates was observed among the collaborating laboratories, participants, garments worn on different occasions, and garment areas sampled. |
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| Keywords: | DNA Transfer Persistence Prevalence Recovery Clothing Activity level Inter-laboratory study |
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