舒芬太尼预处理对大鼠肠道缺血再灌注后氧自由基介导性损伤的影响

目的:观察舒芬太尼预处理对大鼠肠道缺血再灌注损伤(IRI)病变组织及血清中丙二醛(MDA)与超氧化物歧化酶(SOD)活性的影响。方法:Wistar大鼠24只,随机平均分为假手术组(Sham组)、肠缺血再灌注组(IRI组)、舒芬太尼预处理组(SUF组)、纳洛酮拮抗组(NLX组)。以夹闭肠系膜上动脉60min再灌注120min制作IRI模型,于再灌注末刮取大鼠肠黏膜组织测SOD和MDA,留取腔静脉血检测血清中SOD和MDA,剪取相同部位4cm肠段检测组织含水率。结果:IRI组组织和血清MDA和组织含水率较Sham组显著升高,分别增加189.8%、285%和13.5%(均P<0.01);组织和血清SOD活性明显下降(73.5±4.3vs29.4±4.1,107±6vs51±5,均P<0.01)。舒芬太尼预处理可使IRI后MDA、组织含水率升高和SOD下降明显减缓,组织和血清中MDA较IRI组分别降低40.9%与53.2%,SOD升高99.6%与68.6%(均P<0.01),但与Sham组比较差异仍有显著性(均P<0.01)。纳洛酮不能完全拮抗舒芬太尼预处理对IRI的保护效应,两组各参数比较差异仍有显著性(均P<0.01)。组织和血清MDA和SODPearson相关系数分别为0.891和0.807。结论:舒芬太尼预处理大鼠肠缺血再灌注损伤可能通过抑制大鼠IRI后脂质过氧化、保护SOD等抗氧化酶活性,在一定程度上减轻IRI引起的氧自由基介导性损害。纳洛酮拮抗舒芬太尼预处理并不能完全地逆转舒芬太尼预处理对受损肠道的保护作用。

Effects of sufentanil pretreatment on intestinal ischemia-reperfusion injury mediated by oxygen free radical in rats

Objective To investigate the effects of sufentanil pretreatment on the levels of malondialdehyde(MDA) and superoxide dismutase(SOD) in tissue and sera after intestinal ischemia-reperfusion injury(IRI) in rats.Methods 24 Wistar rats were randomly divided into 4 groups:sham group,IRI group,sufentanil pretreatment group(SUF group),and naloxone antagonist group(NLX group).IRI model was made by clamping the superior mesenteric artery(SAM) for 60 min and then released for 120 min,then intestinal mucosa was scraped,and the levels of SOD and MDA in collected sera from superior vena cava and intestinal mucosa at the end of reperfusion were measure,and 4 cm bowel was clipped for detecting water rate.Results Levels of MDA in tissue and sera,and tissue water rate in IRI group were increased by 189.8%,285%,and 13.5% respectively as compared to those in sham group(P < 0.01);while the activities of SOD in tissue and sera were decreased [(73.5 ± 4.3) vs(29.4 ± 4.1),(107 ± 6) vs(51 ± 5),P < 0.01].Sufentanil pretreatment on IRI could down-regulate the increase of MDA and tissue water rate,and the decrease of SOD;the levels of MDA in tissue and sera were decreased by 40.9% and 53.2%,while the levels of SOD in tissue and sera were increased by 99.6% and 68.6%(P < 0.01) as compared to those in IRI group,and were still significantly different from those in sham group(P < 0.01).Naloxone antagonized sufentanil couldn’t fully reverse the protection of sufentanil pretreatment on IRI,parameters of the two groups still had significant differences(P < 0.01).Pearson correlation coefficients of the two MDA and two SOD were 0.891 and 0.807.Conclusions Sufentanil pretreatment could reduce intestinal I/R damage mediated by OFR possibly by inhibiting lipid peroxidation after IRI,protecting SOD and other antioxidant enzymes.Naloxone pretreatment did not completely reverse the protective effect of sufentanil pretreatment on intestinal damage.