| 小鼠原发性肝癌中胰岛素样生长因子2和H19的表达及意义 |
| |
| 引用本文： | 文国容,金海,徐尚福,杨媛,计蓓,周姝,陈萍,庹必光.小鼠原发性肝癌中胰岛素样生长因子2和H19的表达及意义[J].遵义医学院学报,2013(5):422-424. |
| |
| 作者姓名： | 文国容 金海 徐尚福 杨媛 计蓓 周姝 陈萍 庹必光 |
| |
| 作者单位： | [1]遵义医学院附属医院消化内科,贵州遵义563099 [2]遵义医学院药理学教研室,贵州遵义563099 [3]贵阳医学院肿瘤医院药剂科,贵州贵阳550001 |
| |
| 基金项目： | 国家自然科学基金项目（NO：81160054）. |
| |
| 摘 要： | 目的 观察IGF2、H19在原发性肝癌中的表达并了解其意义.方法 建立原发性肝癌模型,模型建立后第20周处死小鼠取肝癌及癌旁组织储存于-80℃备用,另取正常小鼠肝脏作对照,用Real time RT-PCR方法检测IGF2、H19 mRNA的变化.结果 与对照组比较,IGF2、H19在癌旁及癌组织中的表达都显著增加,在癌组织中增加更为显著P＜0.05.结论 IGF2、H19的异常变化与小鼠原发性肝癌的发生发展有关.
|
| 关 键 词： | 胰岛素样生长因子2 H19 小鼠 原发性肝癌 |
Effects of Insulin-like growth factor 2 and H19 in mouse hepatocellular carcinoma |
| |
| Wen guorong,Jin hai,Xu shangfu,Yang yuan,Ji bei,Zhou shu,Chen ping,Tuo biguang.Effects of Insulin-like growth factor 2 and H19 in mouse hepatocellular carcinoma[J].Acta Academiae Medicine Zunyi,2013(5):422-424. |
| |
| Authors: | Wen guorong Jin hai Xu shangfu Yang yuan Ji bei Zhou shu Chen ping Tuo biguang |
| |
| Institution: | 1. Department of Gastroenterology, The Affiliated Hospital of Zunyi Medical University, Guizhou Zunyi 563099, China; 2. Department of Pharmacology of Zunyi Medical University, Guizhou Zunyi 563099, China; 3. Department of Pharmacy, Tumor Hospital of Guiyang Medical College, Guizhou Guiyang 550001, China) |
| |
| Abstract: | Objective To investigate the effects of IGF2 and H19 in mouse hepatocellular carcinoma and discuss its functional mechanism. Methods C57BL/6J mice were given diethyl nitrosamine （DEN） and CC14 to induce hepatocellular carcinoma. At 20 weeks, normal liver tissue, DEN - treated peri - cancerous and cancer tissue were collected to isolated total RNA. Expression of IGF2 and H19 were ex- amined by real -time RT -PCR. Results Compared to the control ,the levels of IGF2 and H19 in model group were significantly increased （ P 〈 0. 05 ）. Conclusion Increased expression of IGF2 and H19 is associated with the development of DEN - induced hepatocellular carcinoma in mice. |
| |
| Keywords: | IGF2 H19 mice Hepatocellular Carcinoma |
| 本文献已被 维普 等数据库收录！ |
|
