32P-磷酸铬-聚-L-乳酸粒子植入治疗裸鼠荷人胰腺癌移植瘤的研究

目的 探讨32p-磷酸铬-聚-L-乳酸(32P-CP-PLLA)粒子瘤体间质给药治疗BALB/c裸鼠荷人胰腺癌( BxPc-3)移植瘤的药效学及全身不良反应.方法 24只荷瘤裸鼠,随机分为4组:空白对照组、37.0、18.5、9.3 MBq组.经瘤体分别给予剂量为0、9.3、18.5和37.0 MBq 32p-CP-P...

Experimental study of 32P-CP-PLLA microparticle on human pancreatic carcinoma in nude mice

Objective To study the therapeutic and toxic effects of³²P-chromic phosphate-poly (L-lactic) acid (³²P-CP-PLLA) microparticle intratumoral administration into BALB/c nude mice bearing BxPc-3 human pancreatic carcinoma. Methods Twenty four nude mice bearing tumors were injected with 0, 9.3, 18.5 and 37.0 MBq ³²P-CP-PLLA microparticle,respectively. The relative tumor growth rates were observed every day, and white blood cells, platelets and body weight were measured. At 14 d after administration, the tumors were removed, histological examination and immunohistochemical analysis were performed. Results The relative tumor growth rates of each treatment group was lower than 40%. Histological examination showed the degenerative necrosis at the site nearby the mircoparticle. Immunohistochemical analysis showed that the Microvessel density (MVD) and the expression of Bcl-2 in treated group were lower than those in control group. In contrast, the expression of bax in treated group were higher than those in control group. The ratio of Bcl-2/Bax protein significantly decreased in the treatment group, which were 3.83±0.43, 0.47±0.13, 1.10±0.32, 2.19±0.57 for 0, 9.3, 18.5 and 37.0 MBq ³²P-CP-PLLA microparticle, respectively (t=2.36-2.77, P<0.05). MVD were 31.2±2.3, 23.8±1.5, 14.8±0.8, 11.0±1.2, respectively. Dose dependence was observed in both HE and IHC staining after 14 d treatment (t=2.30-2.57,P<0.05). Conclusions Intratumoral injection of ³²P-CP-PLLA microparticle might be a safe, easy and effective radionuclide interventional therapy for pancreatic carcinoma.