急性肺栓塞大鼠血管内皮功能动态观察

目的探讨急性肺栓塞大鼠血浆内皮素(ET)、NO水平的动态变化,了解血管内皮功能的改变。方法雄性SD大鼠32只,随机分为对照组、栓塞24h组、栓塞1周组、栓塞2周组,每组8只。医用明胶海绵微粒制备大鼠急性肺栓塞模型,测定血浆ET、NO,HE染色。结果与对照组比较,栓塞24h组、栓塞1周组、栓塞2周组大鼠血浆ET水平呈持续升高状态,NO水平呈持续降低状态。光镜下观查,栓塞2周组大鼠肺动脉内明胶海绵多数溶解。结论急性肺栓塞大鼠血浆ET和NO水平与肺血管内皮功能损伤有关。

The dynamic changes of rat blood vessel endothelium function in acute pulmonary embolism

Objective To observe the dynamic changes of rat blood endothelin(ET)and nitric oxide(NO)levels in order to explore the functional change of rat blood vessel endothelium in acute pulmonary embolism(PE).Methods Male SD rats were injected with medical gelfoam microspheres via jugular vein to prepare PE model.Thirty-two rats were randomized into four groups of 8 rats each:control group,embolism for 24 hours group,embolism for 1 week group and for 2 weeks group.Blood ET,NO were examined at the time of sacrifice.Lung tissue was sliced and stained with HE to observe the embolism of pulmonary artery.Results Compared with control group,in embolism for 24 hours group,embolism for 1 week group and for 2 weeks group,the blood ET was increased significantly and blood NO was reduced significantly.Most of the the gelfoam dissolved at week 2.Conclusions The elevation of blood ET and reduction of NO in rats after acute PE were related to the injury of blood vessel endothelium.