白介素-8对兔腹主动脉粥样硬化狭窄的影响

目的 观察白介素-8(IL-8)水平对兔腹主动脉粥样硬化狭窄的影响.方法 新西兰大白兔36只随机分为实验组、IL-8单克隆抗体治疗组、对照组.实验组和治疗组动物通过穿刺股动脉送入球囊拉伤腹主动脉.治疗组动物通过耳缘静脉注射IL-8单克隆抗体进行干预.对照组动物只进行股动脉穿刺和留置鞘管,不进行球囊扩张拉伤.分别于实验前,实验后4 h、1 d、3 d,1 w、2 w、4 w,耳缘静脉采血4 ml,检测三组动物血清IL-8水平,4 w后处死动物进行病理组织学检查,应用光学显微镜和计算机病理图像分析系统分析测定以下指标:内膜中膜厚度、面积,计算血管狭窄程度.结果 实验组动物球囊损伤4 h后IL-8水平即开始升高,术后1 d达到峰值,持续增高4 w,且明显高于治疗组和对照组.治疗组和对照组动物IL-8水平无上述变化规律.实验组动物腹主动脉明显狭窄,组织学检查内膜面积和中膜面积、血管狭窄程度等指标大于治疗组和对照组(P<0.01),治疗组管腔狭窄,对照组动物管腔无明显变化,治疗组与对照组比较无显著性差异(P>0.05).相关分析显示,IL-8水平与内膜厚度、内膜面积、中膜厚度、中膜面积等呈正相关(相关系数r分别为0.894,0.783,0.801,0.912,均P<0.01).结论 IL-8水平高表达引起腹主动脉粥样硬化狭窄的产生,经IL-8单克隆抗体治疗后动脉狭窄程度明显减轻.IL-8水平与动脉狭窄程度呈正相关,是发生腹主动脉粥样硬化狭窄的独立危险因素.

The effects of interleukin-8 on atherosclerosis of abdominal aorta in rabbits

Objective To observe the effects of interleukin-8 (IL-8) on atherosclerosis (AS) of abdominal aorta in rabbits. Methods Thirty-six New Zealand white rabbits were randomly divided into experiment, treatment and control groups, each group 12 cases. Rabbits in experiment and treatment groups were injuried in abdominal aorta by balloon inflation after punctured in femoral artery. Monoclonal antibody of IL-8 was injected by venous in rabbits of treatment group. Rabbits in control group were punctured only in femoral artery. Peripheral blood was collected before experiment and 4h, 1,3d, 1, 2, 4 w later. The level of IL-8 was measured by enzyme linked immunosorbent assay ( ELISA) respectively. The luminals of abdomial aorta in three groups were observed 4 w later. Histopalhologic examination was performed. Such contents as follows were assayed by light microscope and computer image analysis system; luminal area, intima and tunica media area, angiostenosis of blood vessel. Results The level of II.-8 was raised in 4 h and achieved to peak in 1 d after balloon inflation in rabbits of experiment group. The higher level of this inflammatory factor lasted 4 w. There was no variation above in treatment and control groups. It was observed that abdominal aorta became stenosis obviously in experiment group. The area of intima and tunica media as well as the extent of stenosis in experiment group were bigger than those in treatment and control groups. There was no statistical difference between treatment and control groups. Correlation analysis indicated that there were positive relations between IL-8 and thickness, area of intima and tunica medias respectively(r =0. 894, 0.783,0. 801,0. 912,P <0. 01). Multiple regression analysis of Logistic indicated that IL-8 was an independent risk for the AS(P<0.01). The high levels of IL-8 leaded to AS in abdominal aorta of rabbits. The severity of stenosis became lessen after intervention by monoclonal antibody of IL-8. Positive corelation was found between the level of IL-8 and severity of arterial stenosis. IL-8 was one of independent risks factor leading occurrence and development of AS. Conclusions The high levels of IL-8 leads to AS in abdominal aorta of rabbits. The severity of stenosis becomes lessen after intervention by monoclonal antibody of IL-8. Positive corelation is found between the level of IL-8 and severity of arterial stenosis. IL-8 is one of independent risks factor leading occurrence and development of AS.