系统性红斑狼疮患者自身抗体联合检测的临床意义

目的：探讨3种抗体联合检测诊断系统性红斑狼疮（SLE）的临床意义。方法选择2012年1月至2014年1月本院收治的SLE患者60例（SLE组），以同期于本院体检健康者60例作为对照组。采用酶联免疫吸附法检测抗双链DNA（dsDNA）抗体，采用免疫印迹法检测抗核小体抗体（AnuA）、抗Sm抗体，比较3种抗体单独检测及联合检测的诊断效能。结果 SLE组AnuA、抗Sm抗体、抗dsDNA抗体及3种抗体联合检测阳性率分别为93．3％、33．3％、46．7％、96．7％，均高于对照组检测结果（ P＜0．05）。3种抗体联合检测具有更高的S L E诊断效能。结论 AnuA、抗ds-DNA抗体、抗Sm抗体联合检测有助于避免SLE的漏诊，从而达到早期诊断、治疗的目的。

Clinical significance of combined detection of autoantibodies in patients with systemic lupus erythematosus

Objective To explore the clinical significance of combined detection of autoantibodies in patients with systemic lupus erythematosus （SLE） .Methods A total of 60 SLE patients ,treated in this hospital from Jan . 2012 to Jan .2014 ,were enrolled as SLE group ,and 60 healthy subjects were enrolled as control group .Enzyme-linked immunosorbent assay was performed to detect anti-double stranded DNA （dsDNA ） antibody ,and Western blot was used to detected ant-nucleosome antibody （AnuA） and anti-Sm antibody .Diagnostic performance of single and com-bined detection of antibodies was compared .Results Positive rates of anti-dsDNA antibody ,AnuA ,anti-Sm antibody and combined detection of the three antibodies were 93 .3% ,33 .3% ,46 .7% and 96 .7% in SLE group ,which were significantly higher than control group（P〈0 .05） .Combined detection of the three antibodies were with higher diag-nostic performance .Conclusion Combined detection of AnuA ,anti-Sm antibody and anti-dsDNA antibody might be helpful to avoid missed diagnosis of SLE .