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| 细胞凋亡信息分子Fas、FasL和NF—kB在氟化物作用后破骨样细胞凋亡过程中的表达 | |
| 引用本文： | Sun YM,Yang FJ,Li YM,Lu B,Zhu M,Qiu MC. 细胞凋亡信息分子Fas、FasL和NF—kB在氟化物作用后破骨样细胞凋亡过程中的表达[J]. 中国医学科学院学报, 2002, 24(5): 491-494 |
| 作者姓名： | Sun YM Yang FJ Li YM Lu B Zhu M Qiu MC |
| 作者单位： | 孙元明（中国医学科学院,中国协和医科大学,放射医学研究所放射生物室,天津,300192） 杨福军（中国医学科学院,中国协和医科大学,放射医学研究所放射生物室,天津,300192） 李雨民（中国医学科学院,中国协和医科大学,放射医学研究所放射生物室,天津,300192） 卢飚（Department of Endocrinology,General Hospital of Tianjin Medical University） 朱梅（Department of Endocrinology,General Hospital of Tianjin Medical University） 邱明才（Department of Endocrinology,General Hospital of Tianjin Medical University） |
| 基金项目： | 国家自然科学基金(39770668,30070828)资助Supported by the National Natural Sciences Foundation of China(39770668,30070828); |
| 摘 要： | 目的：研究氟化物作用后破骨细胞样细胞凋亡过程中细胞凋亡配体Fas、FasL（Fas Ligand)和核因子NF－kB的表达变化,方法：在培养基中分别加入不同浓度的氟化钠培养破骨样细胞,以免疫组织化学染方法观察细胞的Fas、FasL和NF－kB表达,结果：Fas、FasL在破骨样细胞表达量随培养基中氟化钠浓度增高而增高,NF－kB的表达量随培养基中氟化钠浓度增高而降低,两者均具有剂量依赖关系,结论：在氟化钠所致的破骨细胞凋亡过程中,Fas、FasL的表达氟化物浓度增高而加强,而核因子NF－kB的表达随氟化物浓度增高而减弱,两者都具有剂量依赖关系。 |
| 关 键 词： | 细胞凋亡 信息分子 Fas FasL NF－kB 氟化物 破骨样细胞 骨质疏松 |
| 修稿时间： | 2002-02-27 |
Expression of Fas,FasL, and NF-kappa B in the process of osteoclast-like cell apoptosis effected by sodium fluoride |
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| Sun Yuan-ming,Yang Fu-jun,Li Yu-min,Lu Biao,Zhu Mei,Qiu Ming-cai. Expression of Fas,FasL, and NF-kappa B in the process of osteoclast-like cell apoptosis effected by sodium fluoride[J]. Acta Academiae Medicinae Sinicae, 2002, 24(5): 491-494 | |
| Authors: | Sun Yuan-ming Yang Fu-jun Li Yu-min Lu Biao Zhu Mei Qiu Ming-cai |
| Affiliation: | Department of Radiobiology, Institute of Radiation Medicine, CAMS, PUMC, Tianjin 300192, China. |
| Abstract: | OBJECTIVE: To detect the changes in the expression of apoptosis signals: Fas, FasL and NF-kappa B in the process of osteoclast-like cell (OLC) apoptosis effected by sodium fluoride. METHODS: After co-culture of osteoclast-like cells with 0, 5, 10 and 15 mg/L sodium fluoride, Fas, FasL and NF-kappa B antibody expressions were detected by immune-histochemistry. RESULTS: The expression of Fas and FasL increased with the concentration of the sodium fluoride, however the expression of NF-kappa B decreased with the concentration of sodium fluoride. CONCLUSION: In the process of OLC apoptosis induced by sodium fluoride, the expression of Fas and FasL increased, and that of NF-kappa B decreased with the concentration of sodium fluoride respectively, and the changes of the expression present a dose-dependent pattern. |
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