梅毒螺旋体感染四种血清学检测方法的临床评价

目的探讨四种梅毒血清学实验诊断方法的准确性及在临床的应用价值。方法应用甲苯胺红不加热血清学试验（TRUST）、酶联免疫吸附试验（ELISA）和胶体金法检测518例性病科患者血清,并用梅毒螺旋体明胶颗粒凝集试验（TPPA）确认。结果 TRUST法灵敏度为58.18%,ELI SA法为99.37%、胶体金法为99.06%;TRUST法特异度为88.5%,ELI SA法为98.50%、胶体金法为100%。结论梅毒检测方法各有优缺点,应根据检验目的合理选用不同的试验方法。

Clinical evaluation of four different serological diagnosis methods of syphilis

Objective To compare the results of four serologic tests of syphilis serum specimens, and evaluate the values of four tests in the diagnosis of syphilis. Methods TRUST, ELISA and Colloidal Gold were employed to detect the serum antibodies against treponema pallidum in 518 patients with syphilis respectively. And the results of above different methods were compared with TPPA as a gold standard. Results The sensitivities of TRUST, ELISA, Colloidal Gold were 58. 18%, 99.37% and 99.06% respectively. The specificity of TRUST, ELISA, Colloidal Gold were 88.5%, 98.50% and 100% respectively. Conclusion Because each serologic diagnosis method of syphilis has advantages and disadvantages, so we should use reasonable method on the basis of clinical objective.