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Developing microsatellite markers for Geodorum eulophioides (Orchidaceae) and cross-species amplification in two sympatric,congeneric species |
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| Authors: | Ting Ying Qi-Fang Geng Dan-Bi Wang Hong Liu Jing Xu Zi-Bing Zhang Zhong-Sheng Wang Yi-Bo Luo |
| Affiliation: | 1. College of Life Sciences, Nanjing University, Nanjing, 210093, China 2. Department of Earth and Environment, Florida International University, Miami, FL, 33199, USA 3. Fairchild Tropical Botanic Garden, Coral Gables, FL, 33156, USA 4. Yachang National Orchid Nature Reserve, Baise, 533209, China 5. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China |
| Abstract: | Geodorum (Orchidaceae) has approximately ten species, and is mainly distributed in tropical Asia. Among them, Geodorum eulophioides Schltr. is of major conservation concern as it has only four known populations, two each found in China and others in Burma. In this study, we developed 18 polymorphic microsatellites markers in G. eulophioides with the number of alleles ranging from two to 17. The expected and observed heterozygosities ranged from 0.046 to 0.918 and from 0.048 to 1.0000, respectively. The cross-species amplification rates for the sympatric G. recurvum (Roxb.) Alston and G. densiflorum (Lam.) Schltr. were 100 and 83.33 %, respectively. These markers developed would be useful for conservation and population genetic studies. |
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