成都地区汉族人群15个STR基因座遗传多态性的研究

目的对成都汉族D3S1358,TH01,D21S11,D18551,PentaE,D55818,D13S317,D7S820,D16SS39,CSF1PO,Penta D,VWA,D8S1179,TPOX,FGA等15个STR基因座的遗传多态性进行群体遗传学研究。方法利用荧光标记复合扩增及毛细管电泳自动荧光检测的方法,对232名无关个体获得15个STR基因座等位基因的分布频率等群体遗传学数据。结果 D3S1358,TH01,D21S11,D18551,PentaE,D55818,D13S317,D7S820,D16SS39,CSF1PO,Penta D,VWA,D8S1179,TPOX,FGA等15个基因座上分别检出等位基因经检验在D3S1358、TH01、D21S11、D18551、PentaE、D55818、D13S317、D7S820、D16SS39、CSF1PO、Penta D、VWA、D8S1179、TPOX、FGA基因座上分别有8,6,16,16,20,9,8,8,8,8,11,12,8,6,18个等位基因。他们的等位基因频率分别在0.002-0.356、0.037-0.498、0.002-0.256、0.002-0.19、0.002-0.168、0.002-0.349、0.002-0.284、0.002-0.371、0.002-0.267、0.002-0.366、0.002-0.328、0.002-0.31、0.024-0.211、0.002-0.519、0.002-0.209之间。15个STR基因座的基因型在调查的群体中的分布符合Hardy-weinberg平衡,累积个人识别率(TDP)大于0.99999999,累积非父排除率(CEP)等于0.999999。结论这15个STR基因座多态性好,灵敏度高,可用于人类遗传分析及法医学中的亲子鉴定和个人识别。

Genetic polymorphism of fifteen STR loci in the Han population of Chengdu

Objective:To investigate the allele frequencies of fifteen short tandem repeats(STR)loci: D3S1358,TH01,D21S11,D18S51,PentaE,D5S818,D13S317,D7S820,D16S539,CSF1PO,Penta D,vWA,D8S1179,TPOX,FGA in Han populat ion in sichuan province.Methods:Totally 232 unrelated individuals from majority population of Han Chinese in Chengdu have been inspected with 15 polymorphism STR loci D3S1358,TH01,D21S11,D18S51,PentaE,D5S818,D13S317,D7S820,D16S539,CSF1PO,Penta D,vWA,D8S1179,TPOX,FGA by means of capillary electrophoresis an...