大鼠原位肝移植后肿瘤坏死因子-α与供肝损伤的关系

目的观察肿瘤坏死因子-α(TNF-α)在肝移植后早期的活性变化及其与供肝损伤的关系。方法建立大鼠肝移植动物模型,观察其生存期外周血ALT变化、大鼠胆汁分泌量及移植肝门静脉血TNF-α活性。结果供肝冷冻保存4h组及对照组移植大鼠存活期超过50d,而冷冻保存8h组大鼠无一长期存活(均<3d);对照组及4h冷冻保存组胆汁分泌量显著多于8h冷冻保存组(P<0.05),8h组ALT水平显著高于对照组及4h组(P<0.05);8h组移植后TNF-α活性显著升高,且术后2h达高峰,而4h组仅在术后2h轻度升高(P<0.05)。结论肝移植早期TNF-α升高程度与供肝损伤程度有关。TNF-α可能源于移植肝的枯否细胞。

Relationship Between Tumor Necrosis Factor and Injuries of Liver Graft after Orthotopic Liver Transplantation in Rats

Objective To study the relationship between tumor necrosis factor-α(TNF-α) levels and ischemia-reperfusion injury of liver grafts after different cold-storage time during the early stage after orthotopic liver transplantation in rats.Methods The survival periods and peripheral serum ALT levels were observed, and the secretion volume of bile as well as TNF-α activities of blood from portal vein of rats with liver grafts were examined.Results All the animals in groups of control and 4 h cold storage survived over 50 d, and group of 8 h cold storage survived less than 3 d. The secretion volume of bile were more in control and 4 h groups than that in 8 h group( P <0.05), and the ALT levels were lower in control and 4 h group than that in 8 h group( P <0.05). The activity of TNF-α was not detected in the control but elevated significantly in the 8 h group, especially 2 h after operation, and only elevated slightly in the 4 h group at the same time( P <0.05).Conclusion The upregulation of TNF-α is involved in the mechanism of ischemia-reperfusion injury of liver graft during the early stage after orthotopic liver transplantation. TNF-α may be originated from Kupffer cells of liver graft.