EphA2蛋白在弥漫性大B细胞淋巴瘤中的表达及其临床意义

目的 探讨EphA2蛋白在弥漫性大B细胞淋巴瘤（DLBCL）中的表达情况及其与临床病理特征和预后的关系。方法 采用免疫组化EnVision法检测72例DLBCL及10例正常淋巴结组织中EphA2蛋白的表达情况，分析DLBCL组织中EphA2蛋白表达与性别、年龄、分期、B症状、乳酸脱氢酶（LDH）、国际预后指数（IPI）及病理分型等临床病理特征的关系，随访DLBCL患者的生存情况并分析中位生存期（OS）和无进展生存期（PFS），同时采用Cox多因素分析影响预后的独立因素。结果 DLBCL组织中EphA2蛋白的阳性表达率为58.3％（42／72），高于正常淋巴结组织的20.0％（2／10），差异有统计学意义（P＜0.05）；EphA2蛋白表达与分期、病理分型有关，而与性别、年龄、B症状、LDH和IPI均无关（P＞0.05）。全组的中位PFS和OS分别为15.5和22.0个月， LDH是影响PFS和OS的独立预后因素，IPI仅是影响PFS的独立预后因素，分期、EphA2蛋白表达均是影响OS的独立预后因素（P＜0.05）。结论 EphA2蛋白在DLBCL中高表达，其表达与分期、病理分型有关，EphA2阳性表达提示预后不佳。

Expression and clinical significance of EphA2 in diffuse large B-cell lymphoma

Objective To investigate the clinical and prognostic significance of EphA2 expression in diffuse large B-cell lym-phoma（DLBCL）. Methods The immunohistochemistry（EnVision method）was applied to detect the protein expression of EphA2 on tissue sections from 72 DLBCL and 10 normal lymph tissues. Then the relationships between EphA2 expression and clinicopathological variables including gender, age, stage, B symptoms, lactate dehydrogenase（ LDH） , international prognostic index（ IPI） and patholog-ical type were analyzed. The DLBCL patients were followed up for medium progression-free survival（PFS）and overall survival（OS）. Multivariate analysis of these prognostic factors was then performed using the Cox proportional hazards. Results Positive expression rate of EphA2 was 58.3%（ 42/72） in DLBCL tissues, higher than 20.0%（ 2/10） of normal lymph tissues（ P＆lt;0.05） . EphA2 expression was related with staging and pathological type, but not with sex, age, B symptoms, LDH and IPI（ P＆gt;0.05） . The median PFS and OS of 72 DLBCL patients were 15.5 and 22.0 months. LDH was an independent prognostic factor for PFS and OS. IPI only was the inde-pendent prognostic factor for PFS, while staging and EphA2 protein expression were independent prognostic factors for OS（ P＆lt;0.05） . Conclusion There was a high expression rate of EphA2 protein in DLBCL, and its expression was related with staging and pathologi-cal type. The positive expression of EphA2 indicated a poor prognosis for DLBCL.