蛋白酶抑制剂对Balb/c小鼠弓形虫病疗效的实验分析

目的:实验分析蛋白酶抑制剂对Balb/c小鼠弓形虫病的疗效,筛选有效的酶抑制剂,拓展临床对弓形虫病防治用药途径。方法:建立弓形虫病(Balb/c)小鼠模型,观察其患病过程,随机分组,给弓形虫病患鼠选用不同的蛋白酶抑制剂进行治疗,分析其疗效。结果:未经酶抑制剂治疗的Balb/c患鼠均在患病后90h内死亡,患鼠腹水涂片检查,弓形虫发育无异常形态变化。使用蛋白酶抑制剂治疗的Balb/c患病小鼠有8只在患病后200h以上死亡,死亡率占26.7%,患鼠腹水检查,弓形虫发育有一定比例的形态异常。有22只患病小鼠被酶抑制剂治愈,治愈率占73.3%。结论:蛋白酶抑制剂对弓形虫发育和入侵细胞有明显影响,对Balb/c小鼠弓形虫病有较好疗效,其中Pepstatin疗效最佳。

The Experimental Analysis of Curative Effect of Proteinase Inhibitors on Balb/c Mouse Toxoplasmosis

Objective:To choose the effective enzyme inhibitors and develop the medicine ways of preventing toxoplasmosis clinically,analyze the curative effect of proteinase inhibitor on Balb/c mouse toxoplasmosis in experiments.Methods:The mouse modles of toxoplasmosis(Balb/c)were established and the course of falling ill was observed.These experimental mice were randomly divided into 4 groups according to use of different proteinase inhibitors.Result:All of the Balb/c mice without treatment of any enzyme inhibitors died 90 hours later,and the abnormal changes in the shape of toxoplasma was not found when the ascitic fruid was examined.From the Balb/c mice treated with enzyme inhibitors only eight Balb/c mice died over 200 hours later and the death rate was 26.7%.The abnormal shape changes in toxoplasma development were found in certain proportion.22 Balb/c mice were cured well by enzyme inhibitor and the cure rate was 73.3%.Conclusion:The proteinase inhibitors have a clear effect on toxoplasma developing and invading cells and also have a better curative effect on Balb/c mouse toxoplasmosis,especially pepstatin has the best curative effect.