Differentiation of human olfactory mucosa mesenchymal stem cells into photoreceptor cells in vitro |
| |
| Authors: | Wen Lu Da Duan Zacharia Ackbarkhan Ming Lu Min-Li Huang |
| |
| Affiliation: | Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China,Department of Neurosurgery, the 163rd Hospital of Chinese PLA, the Second Affiliated Hospital of Hunan Normal University, Changsha 410003, Hunan Province, China,Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China,Department of Neurosurgery, the 163rd Hospital of Chinese PLA, the Second Affiliated Hospital of Hunan Normal University, Changsha 410003, Hunan Province, China and Department of Ophthalmology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China |
| |
| Abstract: | AIM: To investigate whether the human olfactory mucosa mesenchymal stem cells (OM-MSCs) can differentiate into photoreceptor cells in vitro.METHODS: Through the olfactory mucosa adherent method, olfactory mucosa was isolated, cultured and identified in vitro among mesenchymal stem cells. The cell surface markers were analyzed by flow cytometry, induced to differentiate into retinal photoreceptor cells in vitro, and the expression of rhodopsin was observed and identified by Immunofluorescence and Western blot methods.RESULTS: OM-MSCs from human were spindle cell-based, and showing radial colony arrangement. OM-MSCs were negative for CD34, CD45 and CD105, but positive for CD73 and CD90. Following induction, a strong positive reaction was produced by photoreceptor specific marker rhodopsin in the cells. |
| |
| Keywords: | human olfactory mucosa mesenchymal stem cells differentiation retinal cells |
|
| 点击此处可从《国际眼科》浏览原始摘要信息 |
|
点击此处可从《国际眼科》下载免费的PDF全文 |
|
