锌缺乏和锌过量对培养长骨细胞周期和细胞凋亡的影响

目的　探讨锌缺乏和锌过量对培养长骨细胞周期和细胞凋亡的影响 ,为阐明锌对骨骼的生长发育影响的机制提供科学依据。方法　用小鼠胚胎长骨体外培养 ,采用流式细胞仪研究细胞周期各个时相所占比例和凋亡细胞百分比 ,进行电镜分析 ,观察细胞的超微结构。结果　缺锌可使细胞周期停滞在 G0 /G1期 ,培养 2 d,缺锌组 G0 /G1期细胞所占百分比 (82 .3% )高于对照组(76.9% ) ,G2 /M期细胞所占百分比 (3.4% )低于对照组 (8.9% ) ;培养 4d,缺锌组 G0 /G1期细胞所占百分比 (88.6% )明显高于对照组 (78.4% ) ,S期细胞所占百分比 (4.3% )明显低于对照组 (1 1 .0 % ) ;缺锌可使凋亡细胞数目增加。电镜观察 ,缺锌组可见大量的凋亡细胞 ,而高锌组则有大量的坏死细胞。结论　锌缺乏可改变培养长骨的细胞周期 ,诱导细胞凋亡 ,但锌过量对细胞周期和细胞凋亡没有影响。

EFFECT OF ZINC DEFICIENCY AND EXCESS ON THE CELL CYCLE AND APOPTOSIS OF MOUSE FETAL LONG BONE CELLS

Objective:The effects of zinc deficiency and excess on cell cycle and cell apoptosis were explored to elucidate the mechanism of zinc action on bone growth and development. Method:The in vitro culture of mouse fetal long bones was used, and the cell cycle and cell apoptosis were measured by flow cytometer. Results:Zinc deficiency made cell cycle arrested at G 0/G 1 phase. After 2 days the cell number of G 0/G 1 phase (82.3%) was larger than control (76.9%),while that of G 2/M phase (3.4%) fewer (8.9%). After 4 days, the cell number of G 0/G 1 phase (88.6%) was larger than control group (78.4%), while that of S phase (4.3%) fewer than control (11.0%). Zn deficiency can induce cell apoptosis. There were many apoptotic cells in zinc deficiency group and necrotic cells in zinc excess group. Conclusion: Zn deficiency can alter cell cycle, and induce cell apoptosis of cultured bones, but Zn excess has no effect.