| CD44V6的表达对大肠癌细胞缝隙连接细胞间通讯的影响 |
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| 引用本文： | 李祖国,丁彦青,邓永键. CD44V6的表达对大肠癌细胞缝隙连接细胞间通讯的影响[J]. 解放军医学杂志, 1998, 23(3): 166-168 |
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| 作者姓名： | 李祖国 丁彦青 邓永键 |
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| 作者单位： | 第一军医大学病理学教研室 |
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| 摘 要： | 应用原位分子杂交，免疫组化，免疫荧光定量分析的方法研究ＣＤ４４Ｖ６在人大肠癌细胞中的表达，应用激光漂白后荧光恢复技术研究ＣＤ４４Ｖ６对大肠癌细胞的缝隙连接细胞间通讯的影响。
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| 关 键 词： | CD44V6 肿瘤转移 大肠癌 癌细胞 细胞间通讯 |
EXPRESSION OF CD44V6 AND ITS EFFECTS ON GAP JUNCTIONAL INTERCELLULAR COMMUNICATION IN HUMAN COLORECTAL CARCINOMA CELLS |
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| Li Zuguo,Ding Yanqing,Deng Yongjian. EXPRESSION OF CD44V6 AND ITS EFFECTS ON GAP JUNCTIONAL INTERCELLULAR COMMUNICATION IN HUMAN COLORECTAL CARCINOMA CELLS[J]. Medical Journal of Chinese People's Liberation Army, 1998, 23(3): 166-168 |
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| Authors: | Li Zuguo Ding Yanqing Deng Yongjian |
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| Affiliation: | Li Zuguo,Ding Yanqing,Deng Yongjian.Department of Pathology,The First Military Medical Uiversity,Guangzhou 510515 |
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| Abstract: | Expression of CD44V6 variant in human colorectal carcinoma HT29 and Lovo cell lines was investigated by in situ hybridization, immunohistochemistry and quantitative immunofluorescenece technique at mRNA and protein level. The effect of CD44V6 variants on gap junctional intercellular communication (GJIC) was studied by fluorescence recovery after photobleaching (FRAP). The result showed that both of HT29 and Lovo cell lines were well expressed in the CD44V6 mRNA and their proteins, but Lovo cells expressed more highly than HT29 cells both at mRNA and protein levels. Moreover, the expressions of CD44V6 were different among Lovo cell lines, part of which expressed more.The expression of CD44V6 variant was related to metastatic potential of human colorectal carcinoma cells,but CD44V6 could not affect the GJIC of HCC cells. |
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| Keywords: | receptor lymphocyte homing neoplasm metastasis colonic neoplasms |
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