云南边境地区HIV-1型病毒分子流行病学调查

目的为了解云南边境地区人类免疫缺陷病毒（HIV）流行和病毒变异情况,特别是与云南边境地区接壤的缅甸、老挝、越南等国家边境地区HIV流行和HIV基因变异的情况。方法从2007年收集的147例云南省边境地区中国、缅甸、越南籍HIV抗体阳性样本中,通过提取血浆病毒RNA,对HIVgag基因进行聚合酶链反应（PCR）扩增,对扩增产品进行核酸测序和序列分析。结果2007年在云南边境地区检出的HIV-1亚型有A1、B、C、B/C、CRF01_AE、CRF07_BC和CRF08_BC亚型;其中A1亚型为云南省首次检出。结论云南边境地区HIV流行情况十分复杂,存在着7个HIV-1亚型,它们是由边境地区的境内外流动人员引入的。

Molecular-epidemiological survey of HIV-1 in the border areas of Yunnan

Objective To understand the prevalence and variations of HIV-1 in the border areas of Yunnan, especially in those areas which are adjacent to Myanmar, Laos and Vietnam. Methods One hundred and forty seven HIV-positive samples which had been collected from Burmese, Vietnamese and Chinese citizens living or working in the border areas of Yunnan in 2007, were analyzed by a two-step approach. First, the RNA virus nucleic acid was extracted from plasma, and gag gene of HIV was amplified with PCR; second, the amplified gag genes were sequenced with nucleotide and analyzed. Results Seven HIV-1 hypotype （ A1, B, C, B/C, CRF01_AE, CRF 07_ BC, CRF08_BC ） were detected,among which hypotype A1 was detected for the first time in Yunnan. Conclusion The HIV-1 prevalence in the border areas of Yunnan is rather complicated, and seven HIV-1 hypotypes have been detected, which are possibly spread to Yunnan from the neighboring countries.