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| 正交实验法优选苦瓜根总皂苷提取工艺 | |
| 引用本文： | 陈林,何秀丽,朱海涛,方念伯.正交实验法优选苦瓜根总皂苷提取工艺[J].医药导报,2012,31(7):920-923. |
| 作者姓名： | 陈林 何秀丽 朱海涛 方念伯 |
| 作者单位： | 1. 湖北医药学院附属太和医院药剂科,湖北十堰442000;湖北中医药大学中药配伍与中药复方教育部重点实验室,武汉430065 2. 湖北医药学院附属太和医院药剂科,湖北十堰,442000 3. 湖北中医药大学中药配伍与中药复方教育部重点实验室,武汉,430065 |
| 摘 要： | 目的 优选苦瓜根中苦瓜总皂苷的最佳提取工艺. 方法 以提取液中苦瓜总皂苷含量为考察指标,分光光度法测定其含量,采用L9(34)正交实验法优选苦瓜根总皂苷的提取工艺. 结果苦瓜根中苦瓜总皂苷较优的提取工艺为提取2次,每次2 h,乙醇浓度60%,乙醇用量8倍,提取温度80 ℃. 结论 优选得到的提取工艺稳定、可行、重复性好,可为苦瓜根总皂苷的提取提供参考. |
| 关 键 词： | 苦瓜根 苦瓜总皂苷 提取工艺 正交实验 |
| 收稿时间： | 2011-12-5 |
| 修稿时间： | 2012-2-3 |
Optimization of Extraction Process for Saponins in the Roots of Momordica charantia.L by Orthogonal Design |
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| CHEN Lin , HE Xiu-li , ZHU Hai-tao , FANG Nian-bo.Optimization of Extraction Process for Saponins in the Roots of Momordica charantia.L by Orthogonal Design[J].Herald of Medicine,2012,31(7):920-923. | |
| Authors: | CHEN Lin HE Xiu-li ZHU Hai-tao FANG Nian-bo |
| Institution: | 1.Department of Pharmacy,Taihe Hospital Affiliated with Hubei Medical University,Shiyan 442000,China;2.The Ministry of Education Key Laboratory of Chinese Herbal Medicines and Traditional Chinese Medicine Compound,Hubei University of Chinese Medicine,Wuhan 430065,China) |
| Abstract: | Objective To optimize the technology of extraction for saponins in the roots of Momordica charantia.L.Methods The total saponins of Momordica charantia.L and Ginsenoside were taken as indexes,which were detected by spectrometric method.And optimum extraction technology for the total saponins was studied by the L9(34)orthogonal test.Results The optimum extraction technology was obtained as extracting twice,two hours for each,with 8 times 60% alcohol at 80 ℃.Conclusion The optimized technology is stable,feasible and reproducible,which provides evidence for total saponins extraction from the roots of Momordica charantia.L. |
| Keywords: | Roots of Momordica charantia L Total saponins of Momordica charantia L Extraction Orthogonal design |
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