HMGB1 A box蛋白对于急性胰腺炎脏器损伤的保护作用

目的 应用高迁移率族蛋白-1(HMGB1)的拮抗剂-A box蛋白治疗小鼠急性胰腺炎模型并观察其疗效.探讨A box蛋白对于急性胰腺炎所致脏器损伤影响的作用机制.方法 以20%L-精氨酸腹腔注射诱导建立小鼠急性坏死性胰腺炎模型,并于建模后应用A box蛋白进行治疗,测定血AST、ALT、LDH、BUN及Cr等生化指标.比较对照组和治疗组小鼠的胰腺、肺、肾、肝的病理变化及两组小鼠的存活率和存活时间.结果 建模后48 h,治疗组小鼠的胰腺、肺组织损伤病理评分较对照组减少,有显著性差异(P<0.05),治疗组肾、肝组织损伤的病变程度较对照组减轻.建模后24 h治疗组的AST值较对照组明显降低(P<0.05),建模后48 h治疗组的AST、ALT、LDH和Cr值较对照组明显降低(P<0.05).治疗组小鼠的存活率(66.7%)明显高于对照组的存活率(26.7%)(P<0.05).结论 A box蛋白对于胰腺炎及其所引发的肺、肾、肝等脏器的损伤有显著的保护作用.可提高重症胰腺炎小鼠的存活率.

Protective effect of HMGB1 A box on organ injury in mice with acute pancreatitis

Objective To observe the curative effect of the HMGBl A box(HMGB1 antagon)in the acute pancreatitis mice model and explore the mechanism of H MGB1 A box in protecting organ from injury induced by acute pancreatitis.Methods Acute necrotizing pancreatitis mice model was in-duced by 20% L-Arg intraperitoneal injection.The recombination HMGB1 A box was used in treat-ment after modeling and all biochemical indicator including the AST,ALT,LDH,BUN and Cr were measured.The pathological change of pancreases,lungs,kidneys and livers were observed,and sur-vival rates and time were compared between the control group and treatment group.Results The pan-creas and lung tissue pathologic score of the A box treatment group decreased more significantly 48 h after modeling as compared with SAP group.The kidney and liver pathological changes of the treat-ment group was milder than in the SAP group.The AST value of treatment group was significantly lower than that of SAP group 24 h after modeling.The value of AST,ALT,LDH and Cr of treat-ment group declined more significantly as cornpared with the SAP group 48 h after modeling.The mice survival rate of treatment group(66.7%)was significantly higher than that of SAP group(26.7%).Conclusion The recombination HMGB1 A box iS remarkably protective to pancreatitis and organ inju-ry and it can raise the survival rate of mice with severe acute pancreatitis.