胎肝来源间充质干细胞的分离、培养与多向分化

目的从胎肝中分离培养间充质下细胞，并研究其牛物学特性。方法用优化的方法从胎肝中分离获得间充质干细胞。利用流式细胞仪分析细胞表型和细胞周期分布，并体外诱导成骨、成脂肪和成肝组织细胞分化。并用染色方法鉴定成骨、成脂肪分化结合形态学方法和RT-PCR方法鉴定成肝组织分化结果。结果从胎肝中分离培养的细胞为成纤维样，贴壁生长．表型相对均一，表面标志为CD90，CD44，CD147，而CD34，CD45，HLA-DR，具有向肝组织分化的潜能，并可向成骨和成脂肪分化。结论从胎肝中可分离培养得到具有多向分化潜能的间充质干细胞。

Isolation,culture and multipotent differentiation of mesenchymal stem cells from human fetal livers

Objective To isolate and culture mesenchymal stem cells (MSCs) from human fetal livers and describe their biological characteristics. Methods MSCs were acquired using an optimized method. Cell cycles and the immunophenotype of the cells were analyzed by flow cytometry. The osteogenic and adipogenic differentiations were induced and identified by specific stainings, and hepatic differentiation by morphology and RT-PCR. Results The target cells derived from human fetal livers adhered to the plate with fibroblast-like morphology, whose surface markers were CD90, CD44, CD147 positive, and CD34, CD45, HLA-DR negtive. In the differentiation study, these cells could be induced to differentiate into osteogenic, adipogenic and hepatocyte-like cells. Conclusion Multipotent MSCs can be isolated and cultured from human fetal livers.