肝细胞色素P450 2E1在实验性肝纤维化组织中的表达

目的 研究肝细胞色素Ｐ４５０ ２Ｅ１在实验性肝纤维化组织中的表达。方法 ８只Ｗｉｓｔａｒ大鼠,建立四氯化碳（ＣＣｌ４）肝纤维化模型,用免疫组织化学方法观察肝纤维化模型肝组织中肝细胞色素Ｐ４５０ ２Ｅ１。结果 正常肝组织内,ＣＹＰ ２Ｅ１表达仅见于中央静脉周围区,主要表达于肝腺泡Ⅲ区,２～３层细胞厚,肝细胞浆和细胞膜可见ＣＹＰ ２Ｅ１表达。在ＣＣｌ４模型肝组织中,ＣＹＰ ２Ｅ１表达的强度增加,分布的

Expression of hepatic cytochrome P450 2E1 in experimental liver fibrosis

Objective To study expression of hepatic cytochrome P450 2E1 in experimental liver fibrosis. Methods Experimental liver fibrosis model of rats was induced by carbon tetrachloride subcutaneous injection, and expression of hepatic cytochrome P450 2E1 was evaluated with immunohistochemistry by using a monoclonal antibody. Results In the normal rats, CYP 2E1 was localized within a rim of hepatocytes two to three cells wide around the terminal hepatic venule in acinar zone 3. The intensity of CYP 2E1 expression increased in the model, the acinar distribution of CYP 2E1 followed closely the distribution of steatosis, sometimes extending through acinar zone 3 to zones 2 and 1, the hepatocytes in the pseudolobule weren't found. The intensity and distribution of CYP 2E1 expression between rats of model differed. In 8 rats, 2 rats were 3, 3 were 2, 3 were 1.Conclusion CYP 2E1 expression was associated with pathogenesis of liver fibrosis. There were genetic polymorphisms of cytochrome P450 2E1 between rats.