| 高脂高糖诱导的 2 型糖尿病大鼠肾脏细胞凋亡的分析 |
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| 引用本文： | 张曙云,沈征. 高脂高糖诱导的 2 型糖尿病大鼠肾脏细胞凋亡的分析[J]. 临床和实验医学杂志, 2005, 4(3): 131-133 |
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| 作者姓名： | 张曙云 沈征 |
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| 作者单位： | 南京军区杭州疗养院,浙江,杭州,310007;南京军区杭州疗养院,浙江,杭州,310007 |
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| 摘 要： | 目的研究分析高脂高糖诱导的2型糖尿病大鼠肾脏细胞凋亡的变化。方法用高糖高脂饲料(10·0%猪油,20·0%蔗糖,5·0%胆固醇,65·0%常规饲料)喂养SD大鼠诱发胰岛素抵抗,然后用小剂量链脲佐菌素(STZ)腹腔注射,诱发2型糖尿病,用TdT介导的脱氧核苷酸切口末端标记法(TdT-mediateddUTPnickendlabel-ing,TUNEL)检测肾脏细胞凋亡,并与常规饮食大鼠(对照组)相比较。结果2型糖尿病大鼠肾脏细胞的凋亡数显著多于对照组(P<0·0001)。结论本研究为2型糖尿病大鼠肾脏细胞的凋亡提供证据,提示凋亡在肾脏进行性损伤中起重要作用,细胞凋亡与糖尿病肾病的发生密切相关。
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| 关 键 词： | 2型糖尿病 糖尿病肾病 细胞凋亡 |
| 文章编号： | 1671-4695(2005)03-0131-03 |
Renal cell apoptosis in diet-indceced rat type 2 diabetes mellitus induced by diets and apoptosis in rat kidney |
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| ZHANG Shu-yun,SHEN Zheng. Renal cell apoptosis in diet-indceced rat type 2 diabetes mellitus induced by diets and apoptosis in rat kidney[J]. Journal of Clinical and Experimental Medicine, 2005, 4(3): 131-133 |
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| Authors: | ZHANG Shu-yun SHEN Zheng |
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| Abstract: | Objective To study the apoptosis in the type 2 diabetes mellitus rats kidney. Methods SD rats were fed with the diets enriched with sucrose(20%)and lard(10%)and cholesterol(5%)to induce insulin resistance.Type 2 diabetes mellitus was developed by intraperitoneal injection in these rats with 25mg/Kg streptozocin(STZ).Rats Kidney cells apoptosis was detected by in situ TUNEL staining. Results The number of apoptosis cells in type 2 diabetes mellitus rats kidney was higher than that in control(P<0.0001). Conclusion The present study provides evidence for apoptosis in type 2 diabetes mellitus rats kidney.It suggests a role for apoptosis in the gradual loss of renal mass and an intimate relationship between apoptosis and diabetic nephropathy. |
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| Keywords: | Type 2 diabetes mellitus Diabetic nephrophathy Cell apoptosis |
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