高效液相色谱法测定雷公藤饮片中雷公藤内酯醇的含量

目的:建立以高效液相色谱(HPLC)法测定雷公藤饮片水提物中雷公藤内酯醇含量的方法。方法:样品分别用氯仿和乙酸乙酯超声萃取,经中性氧化铝柱洗脱分离后,在流动相为乙腈-水(23∶77)、流速为1.0ml/min、检测波长为218nm条件下测定含量并进行比较。结果:雷公藤内酯醇检测浓度线性范围为2.74～96μg/ml(r=0.9999),平均回收率分别为94.78%(RSD=1.57%)、98.2%(RSD=1.7%)。结论:2种含量测定方法均可用于雷公藤内酯醇的质量控制,尤以乙酸乙酯处理样品的方法分离、测定效果较好。

Content Determination of Triptolide in Tripterygium Wilfordii Cut Crude Drugs by HPLC

OBJECTIVE:To develop a RP-HPLC method for the determination of triptolide in tripterygium wilfordii cut crude drugs.METHODS:Samples were extracted with chloroform and acetic ether by ultrasound and underwent neutral alu-mina column elution and isolation,the content determination was performed with acetonitrile-water(23∶77)as mobile phase at a flow rate of1.0ml/min and detection wavelength at218nm.RESULTS:The linear detection concentration range of trip-tolide alcohol was2.74～96μg/ml(r=0.9999),and the average recovery were94.78%(RSD=1.57%)and98.2%(RSD=1.7%),respectively.CONCLUSION:Both methods are applicable for the quality control of triptolide alcohol,yet the separation and determination outcome were better for the sample treated with acetic ether.