| 抗人肝癌导向药物的制备及其分析 |
| |
| 引用本文： | 黄贤明 魏曙光. 抗人肝癌导向药物的制备及其分析[J]. 免疫学杂志, 1994, 10(1): 40-43 |
| |
| 作者姓名： | 黄贤明 魏曙光 |
| |
| 作者单位： | 北京协和医院细胞基因室，中国协和医科大学基础医学研究所 |
| |
| 摘 要： | 本文报道了肝癌单克隆抗体Ａ２４－表阿霉素结合物的制备及其对肝癌细胞ＳＭＭＣ－７７２１的体外杀伤作用。先用高碘酸钠把葡聚糖氧化成多醛基葡聚糖，再以此为中介体连接单抗与表阿霉素。结合物中抗体与药物摩尔比为１：４０。单抗活性保存良好。该结合物对肝癌细胞的毒性显著高于游离药物及无关抗体结合物，而对非靶细胞的毒性则明显低于游离药物。说明结合物对肝癌细胞有较强的选择性杀伤作用，有可能用于肝癌导向治疗。
|
| 关 键 词： | 单克隆抗体 表阿霉素 抗癌 肝肿瘤 |
Preparation and in Vitro Cytotoxicity of McAb-epirubicin Conjugateagainst Human Hepatoma |
| |
| Huang Xianming, Wei Shguang,Fan qi,He Lun. Preparation and in Vitro Cytotoxicity of McAb-epirubicin Conjugateagainst Human Hepatoma[J]. Immunological Journal, 1994, 10(1): 40-43 |
| |
| Authors: | Huang Xianming Wei Shguang Fan qi He Lun |
| |
| Abstract: | A conjugate of epirubicin with murine McAb A24 against human hepatoma was prepared and its cytotox-icty to hepatoma cell line SMMC-7721 was studied. Dextran T-40 was oxidized to polyaldehyde-dextran (PAD)withsodium periodate. Eprrubicin was then linked covalently with A24 using PAD as a multivalent carrier. The conjugate (A24:epirubicin molar rate,1:40) retained full antibody activity. The cytotoxicity of the conjugate against hepatomacell line SMMC-7721 was significantly higher than that of free epirubicin or normal murine immunoglobulin conjugate. The cytotoxicity of the conjugate was significantly lower than that of free epirubicin against non-target cell Hep- 2.Ourresult suggested that the conjugate exhibited highly selective cytotoxicity to hepatoma cells. |
| |
| Keywords: | McAb. Epirubicin. Immunoconjugate. Hepatoma |
| 本文献已被 CNKI 维普 等数据库收录！ |
