外周血造血干/祖细胞动员与采集时动态快速监测造血祖细胞的意义

为了获得高效的外周血干/祖细胞采集,探索一种简便、快速的外周血干/祖细胞监测方法，采用Sysmex XE-2100血细胞分析仪的幼稚细胞信号（IMI）检测通道识别和计数外周血造血祖细胞（HPC）。对25例行异基因外周血造血干细胞移植动员的供和11例自体外周血干细胞动员的患的外周血造血干/祖细胞进行了动态观察。于动员过程中取外周血进行HPC，CD34^ 细胞和CFU-GM的检测，对采集物也进行上述检测。结果表明：在外周血标本中HPC与CD34^ 细胞和CFU-GM二间均呈良好的正相关性。所有检测病例外周血CD34^ 细胞与HPC同时上升，同时达高峰。供的峰值出现在动员的第5天，快速升高晚于白细胞。而患外周血干/祖细胞的快速升高早于白细胞。采集物中HPC与CD34^ 细胞和CFU-GM呈正相关性。采集当日外周血中HPC和CD34^ 细胞计数与采集所得CD34^ 细胞数量亦具有良好的线性相关。结论：造血祖细胞的监测是一种快速、简便又经济的监测外周血干细胞采集时机和预测成功采集的可靠指标。

Significance of Monitoring Hematopoietic Progenitor Cells in Peripheral Blood Stem Cell during Mobilization and Harvest

To achieve efficient peripheral blood stem cell harvest (PBSCH), a simple method to monitor peripheral blood stem/progenitor cells was evaluated. The Sysmex XE 2100 hematology analyzer with an immature information(IMI) channel was used to identify and count the hematopoietic progenitor cell (HPC). Twenty five donors mobilized with G CSF in allogeneic and 11 patients in autologous peripheral blood stem cell transplantation (allo PBSCT and auto PBSCT) were involved. The HPC, CD34 + cell and CFU GM in the peripheral blood and leukapheresis samples were detected during mobilization and harvest. The results showed that HPC amount had a positive correlation with both the CD34 + cell and CFU GM in the peripheral blood. The peripheral blood hematopoietic stem/progenitor cells in allo PBSCT donors remarkably increased on day 5 of the mobilization, followed the leukocytes increased. However, a fast increase of hematopoietic stem/progenitor cells was earlier than leukocytes in the peripheral blood . The HPC positively correlated with the CD34 + cell or CFU GM in the PBSCH. On the days of collection, the count of HPC and CD34 + cell in peripheral blood was highly correlated with the CD34 + cell yield. It is concluded that HPC as an estimate of progenitor cells in collected blood sample could be used to determine the optimal time of PBSCH and minimize the risk of missing an adequate harvest.