EGFR、P16、Ki67和P53在宫颈鳞状上皮内瘤变和宫颈鳞状细胞癌中表达的临床意义

目的探讨EGFR、P16、Ki67和P53在宫颈病变组织中的表达及临床意义。方法应用免疫组化SP法检测84例宫颈鳞状上皮内瘤变（CIN）和30例宫颈鳞癌中EGFR、P16、Ki67和P53的表达，另选取20例正常宫颈组织作为对照。结果EGFR、P16、Ki67和P53在正常宫颈组织中阳性率分别为10．00％、0、5．00％及5．00％；在CIN中阳性率分别为33．33％、82．14％、54．76％及47．62％；在宫颈鳞癌中阳性率分别为56．67％、100．00％、86．67％及76．67％，差异均有统计学意义（P〈0．05）。EGFR、P16、Ki67和P53表达强度均随宫颈病变的进展而增加，表达均有统计学意义（P〈0．05）。EGFR、P16、Ki67和P53表达在CIN和鳞癌中两两比较均呈显著性正相关（P〈0．05）。结论EGFR、P16、Ki67和P53可能参与了宫颈癌病变的发生及发展，联合检测四者可作为CIN分级与鳞癌诊断的重要参考指标。

Expression and its clinical significance of EGFR P16 Ki67 and P53 in cervical intraepithelial neoplasia and cervical squamous cell carcinoma

Objective To investigate the clinicopathologic significance of the expression of EGFR,P16,Ki67 and P53 proteins in cervical lesion tissues. Methods To detect the expression of EGFR,P16,Ki67 and P53 proteins in 20 nor- mal uterine cervix, 84 cervical intraepithelial neoplasia （CIN） and 30 cervical squamous cell carcinoma by immunohis- toehemical method. Results The positive expression rates of EGFR,P16,Ki67 and P53 were 10.00%, O, 5.00% and 5.00% in normal uterine cervix. The positive expression rates of EGFR,P16,Ki67 and P53 were 33.33% ,82.14%, 54.76% and 47.62% in CIN. The positive expression rates of EGFR,P16,Ki67 and P53 were 56.67% ,100.00%, 86.67% and 76.67% in cervical squamous cell carcinoma. The significant differences statistically of EGFR,P16 ,Ki67 and P53 proteins expression in normal uterine cervix, CIN and cervical squamous cell carcinoma were observed（P 〈 0.05）. The positive expression rates of EGFR,PI6 ,Ki67 and P53 increased with the progression of cervical neoplasia. All groups had statistical difference （P 〈 0.05 ）. EGFR,P16 ,Ki67 and P53 expression was positively associated with each other （P 〈 0.05）. Conclusion EGFR,P16,Ki67 and P53 expression may be involved in the occurrence and devel- opment of cervical lesions.Combined detection of the four proteins can be used as the CIN classification and diagnosis of cervical squamous cell carcinoma of the important reference index.