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| Apoptin基因诱导膀胱癌耐药细胞凋亡的研究 | |
| 引用本文： | 刘迎,杨江根,肖克峰. Apoptin基因诱导膀胱癌耐药细胞凋亡的研究[J]. 江西医学院学报, 2008, 48(2): 5-7 |
| 作者姓名： | 刘迎 杨江根 肖克峰 |
| 作者单位： | 暨南大学附属深圳市人民医院泌尿外科,广东深圳 518020 |
| 摘 要： | 目的观察Apoptin基因对膀胱癌耐药细胞株EJ／MDR细胞的杀伤效果，探讨其可能的机制。方法用脂质体将Apoptin基因导人EJ／MDR细胞，通过RT-PCR法检测ApoptinmRNA的表达，同时用SABC免疫组化法分析Apoptin和p53的表达。采用细胞计数法检测细胞生长抑制率，TUNEL法标记凋亡细胞，流式细胞仪检测细胞周期的变化。结果转入Apoptin基因后，EJ／MDR细胞的生长明显受抑制（P％0．05）。细胞周期分析可见凋亡峰，凋亡率35％。细胞中可见Apoptin阳性表达（P〈0．05），p53表达无显著性差异（P〉0．05）。凋亡细胞在荧光显微镜下形成凋亡小体。结论转入Apoptin基因可显著促进EJ／MDR细胞的凋亡，Apoptin诱导的凋亡不依赖功能性p53的生成。 |
| 关 键 词： | Apoptin基因 膀胱肿瘤 多药耐药 凋亡 |
| 文章编号： | 1000-2294(2008)02-0005-03 |
| 修稿时间： | 2007-09-29 |
Acceleration of Apoptosis by Transfection of Apoptin Gene in Multi-drug Resistant Bladder Cancer Cells |
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| LIU Ying,YANG Jiang-gen,XIAO Ke-feng. Acceleration of Apoptosis by Transfection of Apoptin Gene in Multi-drug Resistant Bladder Cancer Cells[J]. Acta Academiae Medicinae Jiangxi, 2008, 48(2): 5-7 | |
| Authors: | LIU Ying YANG Jiang-gen XIAO Ke-feng |
| Affiliation: | LIU Ying,YANG Jiang-gen,XIAO Ke-feng(Department of Urology,the People's Hospital of Shenzhen,Ji'nan University,Shenzhen 518020,China) |
| Abstract: | Objective To observe the effects of Apoptin gene on killing MDR bladder cancer cells and illustrates its mechanisms.Methods Apoptin gene was transfected into MDR bladder cancer cells by liposome.The mRNA of Apoptin and bcl-2 were detected by RT-PCR.The protein of Apoptin and p53 were detected by SABC immunohistochemistry.The growth rate of human bladder cancer cells were studied by constructing the growth curve and TUNEL,cell apoptosis was observed by flow cytometry.Results The expression of Apoptin mRNA wa... |
| Keywords: | apoptin gene bladder cancer multi-drug resistant apoptosis |
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