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| ELISA测定血清半胱氨酸蛋白酶抑制剂C | |
| 引用本文： | 单桂芬,林伟,荣墨克,张静春. ELISA测定血清半胱氨酸蛋白酶抑制剂C[J]. 中国实验诊断学, 2010, 14(1): 105-106 |
| 作者姓名： | 单桂芬 林伟 荣墨克 张静春 |
| 作者单位： | 1. 吉林大学第二医院,检验科,吉林,长春130041 2. 吉林大学中日联谊医院 |
| 摘 要： | 目的建立ELISA方法测定血清半胱氨酸蛋白酶抑制剂C。方法以双抗体夹心ELISA方法测定血清半胱氨酸蛋白酶抑制剂C。结果该方法的线性范围可达100mg／L。参考值范围为：男，1．81±0．73mg／L。女，1．72±0．69mg／L。回收率为：99．1±3．1％。批内和批间分别为：5．8±0．5％、7．3±O．7％。结论该方法操作简便，快速，准确，适于临床常规应用。 |
| 关 键 词： | ELISA方法 半胱氨酸蛋白酶抑制剂C 肾功能 |
The Method for Determination of Cystatin C by ELISA |
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| SHAN Gui-fen,LIN Wei,RONG Mo-ke,et al.. The Method for Determination of Cystatin C by ELISA[J]. Chinese Journal of Laboratory Diagnosis, 2010, 14(1): 105-106 | |
| Authors: | SHAN Gui-fen LIN Wei RONG Mo-ke et al. |
| Affiliation: | SHAN Gui-fen,LIN Wei,RONG Mo-ke,et al.(The Second Hospital of JiLin University,Changchun 130041,China) |
| Abstract: | Objective Th a enzyme-linked inununoserbent assay （ELISA） for measuring eystatin C. Methods e7 ystatin C in serum was measured by sandwieh-type ELISA. Results The linearity of method for up to 100 mg/L. The reference value range was： males, 1.81 ±0.73 mg/L. females, 1.72± 0.69 mg/L. The analytieal recovery was 99.1 ±3.1%. The mean eoeffieients of variation were 5.8±0.5% within-assay and 7.3±0.7% between-assay,respectively. Conclusion The method was simple,rapid and aeeu- rate to operate. It is suitable for routine elinieal labtatory use. |
| Keywords: | ELISA Cystatin C kidney function |
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