| B7-1基因抑制小鼠前胃癌细胞增殖活性的实验研究 |
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| 引用本文： | 朱林,熊俊,郑启昌. B7-1基因抑制小鼠前胃癌细胞增殖活性的实验研究[J]. 中华肿瘤防治杂志, 2004, 11(2): 131-133 |
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| 作者姓名： | 朱林 熊俊 郑启昌 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院普外科,湖北,武汉,430022;华中科技大学同济医学院附属协和医院普外科,湖北,武汉,430022;华中科技大学同济医学院附属协和医院普外科,湖北,武汉,430022 |
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| 摘 要： | 目的 :研究B7 1基因对小鼠前胃癌细胞 (MFC)增殖活性的抑制作用 ,探讨其在肿瘤免疫中的作用。方法 :将B7 1基因转染MFC细胞后 ,用MTT法和流式细胞仪 (FCM )分别测定转染细胞增殖活性的变化 ,并将已转染B7 1的MFC细胞种植到裸鼠皮下 ,研究其对体内成瘤的影响。结果 :转染B7 1基因的MFC细胞增殖活性受到抑制 ,P <0 0 5。结论 :B7 1基因对MFC细胞增殖活性有抑制作用。
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| 关 键 词： | 胃肿瘤/病理学 基因 转染 鼠科 |
| 文章编号： | 1009-4571(2004)02-0131-03 |
| 修稿时间： | 2003-02-19 |
Study on the suppression of cell proliferation of MFC transfected by B7-1 gene |
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| ZHU Lin,XIONG Jun,ZHENG Qi chang. Study on the suppression of cell proliferation of MFC transfected by B7-1 gene[J]. Chinese Journal of Cancer Prevention and Treatment, 2004, 11(2): 131-133 |
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| Authors: | ZHU Lin XIONG Jun ZHENG Qi chang |
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| Affiliation: | ZHU Lin,XIONG Jun,ZHENG Qi chang Department of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,P.R.China |
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| Abstract: | OBJECTIVE:To investigate the suppression effect of B7 1 gene on proliferous characteristics of MFC cells.METHODS:MFC was transfected by B7 1 gene with the liposomes induced.The proliferation was assayed by MTT and FCM.Then the MFCs transfected by B7 1 were transplanted into subcutaneous of nude mice.RESULTS:The proliferation of MFCs transfercted by B7 1 was suppressed, P <0 05.CONCLUSIONS:The transfer of B7 1 gene can effectively suppress the proliferation of MFC cells. |
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| Keywords: | stomach neoplasms/pathology gene transfection muridae |
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