氯胺酮、咪达唑仑及丙泊酚对发育期神经元细胞内钙的影响

目的：探讨氯胺酮、咪达唑仑及丙泊酚对大鼠发育期海马神经元细胞内钙浓度的影响．方法：将原代培养第5日的大鼠海马神经元用10μmol／L的Ca2＋指示剂Fluo-4共孵育30min洗涤后，分别加入150μmol／L氯胺酮，咪达唑仑3μmol／L，丙泊酚10μmol／L，采用激光共聚焦显微镜选定多个细胞分别测定荧光强度的变化．结果：氯胺酮使体外培养第5日的海马神经元代表钙浓度的荧光强度明显下降（987±307）vs（766±226），P〈0．05]，咪达唑仑，丙泊酚均使体外培养5d的海马神经元荧光强度明显升高（1707±514）vs（2663±572），（1057±353）vs（1749±708），P〈0．05]．结论：阻滞NMDA受体的氯胺酮降低发育期海马神经元细胞内钙浓度，而兴奋GABA。受体的咪达唑仑，丙泊酚则会升高细胞内钙浓度．

Effect of ketamine,midazolam and propofol on intracellular Ca~(2+) in developing neurons

AIM ： To investigate the effect of ketamine, midazo- lam and propofol on intraceflular Ca2＋ in developing primary hippoeampus neurons of rats. METHODS： On the fifth day, the cultured hippocampal neurons were co-incubated with 10 μmol/L Fluo-4 AM for 30 min at 37℃. Excess dye was removed with three rinses of DMEM. Fluorescence imaging of intracellular Ca2. was performed to detect a number of neurons selected when the hippoeampal neurons were exposed to 150μmol/L ketamine, 3 μmol/L midazolam or 10 μmol/L propofol respectively. RESULTS： After exposed to 150 μmolfL ketamine, the intracel- lular calcium concentration of the developing neurons decreased significantly and fluorescence intensity of the neurons decreased from （987 ± 307） to （766 ± 226） （P 〈 0.05 ）. When exposed to 3 μmol/L midazolam or 10 μmol/L propofol, the cytosolie Ca2 ＋of the neurons increased significantly and fluorescence intensity of the neurons exposed to midazolam increased from（ 1707 ± 514 ） to （2663 ± 572） （ P 〈 0.05 ）. Fluorescence intensity of the neurons exposed to propofol increased from（ 1057 ±353 ） to （1749 ±708） （ P 〈 0.05 ）. CONCLUSION ： Ketamine decreases the intracel- lular Ca2＋ of rat developing primary hippocampus neurons while midazolam and propofol may increase the intracellular Ca2 ＋ of the neurons.