安宫牛黄丸对脂多糖致急性肺损伤大鼠肺组织Nrf2蛋白表达的影响

目的 探讨安宫牛黄丸对脂多糖引起的急性肺损伤的保护机制.方法 将40只SD大鼠按随机数字表法分为对照组(Control组,n=10)、脂多糖组(LPS组,n=10)、脂多糖+安宫牛黄丸组(LPS+AG组,n=10)和安宫牛黄丸组(AG组,n=10).采用一次性腹腔注射脂多糖30 mg/kg建立大鼠急性肺损伤模型.分别检测各组大鼠肺湿重/干重比、支气管肺泡灌洗液蛋白浓度和白细胞计数、肺组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)和肿瘤坏死因子α(TNF-α)含量、肺组织内转录因子NF-E2相关因子2(Nrf2)水平.结果 LPS+AG组肺组织SOD活性为(325.67±7.85)kU/g,明显高于LPS组的(298.75±6.25)kU/g,差异有统计学意义(P<0.05).MDA、INF-α含量分别为(1.89±0.35)μmol/L、(35.21±3.12)ng/g,均低于LPS组的(2.78±0.41)μmol/L、(41.52±4.87)ng/g,差异均有统计学意义(P<0.05).LPS+AG组肺组织Nrf2蛋白表达为(0.87±0.19),明显高于LPS组的(0.34±0.11),差异有统计学意义(P<0.05).结论 安宫牛黄丸对脂多糖诱发的急性肺损伤具有保护作用,可能与其能激活肺组织Nrf2表达有关.

Effects of Angong Niuhuang Wan on expression of Nrf2 protein in lung tissue of rats with LPS-induced acute lung injury

Objective To investigate the protective mechanism of Angong Niuhuang Wan (AG) on lipopoly-saccharide-induced (LPS-induced) acute lung injury (ALI) in rats. Methods Fourty rats were randomly divided into four groups:control group (n=10), LPS group (n=10), LPS+AG group (n=10) and AG group (n=10). The ALI rat models were established by intraperitoneal injection of LPS 30 mg/kg. The lung wet/dry weight ratio, total protein concentra-tion and WBC count in brochoalveolar lavage fluid (BALF), lung tissue superoxide dismutase (SOD) activity, malonal-dehyde (MDA) and tumor necrosis factor α (TNF-α) content, lung tissue nuclear factor erythroid 2-related factor 2 (Nrf2) levels were respectively examinated among the four groups. Results The SOD activity in lung tissue of LPS+AG group was (325.67±7.85) kU/g, which was significantly higher than that of LPS group of (298.75±6.25) kU/g, and the difference was statistically significant (P<0.05). The content of MDA and INF-αof LPS+AG group were respectively (1.89±0.35)μmol/L and (35.21±3.12) ng/g, which were significantly lower than (2.78±0.41)μmol/L, (41.52±4.87) ng/g of LPS group, and the differences were statistically significant (P<0.05). The expression of Nrf2 protein in lung tissue of LPS+AG group was (0.87±0.19), which was significantly higher than that of LPS group (0.34±0.11), and the difference was statistically significant (P<0.05). Conclusion Angong Niuhuang Wan has protective effects on LPS-induced ALI, and it may be related to the activation of Nrf2 expression in lung tissue.