| 微粒子酶联免疫吸附法测定西罗莫司血药浓度的方法学评价 |
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| 引用本文： | 董振南,贾兴旺,王玲,田亚平. 微粒子酶联免疫吸附法测定西罗莫司血药浓度的方法学评价[J]. 军医进修学院学报, 2006, 27(5): 366-367 |
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| 作者姓名： | 董振南 贾兴旺 王玲 田亚平 |
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| 作者单位： | 解放军总医院,生化科,北京,100853 |
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| 摘 要： | 目的:通过西罗莫司(S irolimous)试剂盒的化学性能测定,评价微粒子酶联免疫吸附法测定的可靠性。方法:用本方法在雅培IMX分析仪上对西罗莫司血药浓度的精密度、回收率、线性、相关性等指标进行评价。结果:本方法的评价表明,批内变异系数分别为2.4%,6.5%,1.8%,批间变异系数分别为8%,10.33%,11.9%,在1.5~30 ng/m l范围内,线性良好。与高效液相色谱法测定相关性良好(r=0.9361)。结论:该方法操作简便、快速、准确,适用于临床上对肾移植患者西罗莫司药谷浓度的监测。
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| 关 键 词： | 西罗莫司 酶联免疫吸附法测定 色谱法 高压液相 |
| 文章编号： | 1005-1139（2006）05-0366-02 |
| 收稿时间： | 2005-11-18 |
| 修稿时间： | 2005-12-27 |
Method evaluation on plasma concentration of Sirolimous by MEIA |
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| DONG Zhen-nan,JIA xing-wang,WANG Lin,TIAN Ya-ping. Method evaluation on plasma concentration of Sirolimous by MEIA[J]. Academic Journal of Pla Postgraduate Medical School, 2006, 27(5): 366-367 |
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| Authors: | DONG Zhen-nan JIA xing-wang WANG Lin TIAN Ya-ping |
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| Affiliation: | Department of Clinical Biochemistry, PLA General Hospital, Beijing 100853, China |
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| Abstract: | Objective:For evaluating reliability of Sirolimous assay reagent,chemical characters were determined.Methods: The precision,linearity and recovery rate and correlation of this method were evaluated.Results:The within-run CV of low,middle and high level were 2.4%,6.5%,1.8% respectively.The between-run CV of low,middle and high level were 8%,10.33%,11.9% respectively.In the range of 1.5-30 ng/ml,result had good linearity.The correlation coefficient of this method with HPLC was 0.9361.Conclusion:The method is simple,quick,accurate and suitable for monitoring the Sirolimous concentration of renal transplant patients. |
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| Keywords: | Sirolimus enzyme-linked immunosorbent assay HPLC |
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