HPLC法测定苍耳子中苍耳子噻嗪双酮苷的含量

目的建立苍耳子中苍耳子噻嗪双酮苷的含量测定方法。方法采用乙醇提取、正丁醇萃取与硅胶柱色谱分离等方法制备苍耳子噻嗪双酮苷;采用HPLC法进行含量测定,色谱条件为:Dia-monsilTM ODS色谱柱(250 mm×4.6 mm,5μm),以质量分数为0.04%的磷酸溶液-乙腈(体积比为90∶10)为流动相,检测波长为254 nm,流速为1 mL.min-1。结果苍耳子噻嗪双酮苷质量浓度在1～100 mg.L-1内线性关系良好(r=0.999 9,n=6),平均回收率为100.2%,RSD为2.3%(n=9)。结论HPLC法适合测定苍耳子中苍耳子噻嗪双酮苷的含量。

Determination of content of xanthiside from fruit of Xanthiunm sibiricum by HPLC

Objective To develop a method for the determination of the content of xanthiside from Xanthiunm sibiricum by HPLC. Methods Xanthiside was obtained by the extraction with 70 % (φ)EtOH and purified by column chromatography. DiamonsilTM ODS column was used for the determination of xanthiside. The mobile phase was acetonitrile and aqua w(H3PO4)=0.04%]with the volume ratio of 10:90 and the UV detection wavelength was set at 254 nm; the flow rate was 1.0 mL·min-1; the column temperature was 40 °C. Results The linear range of xanthiside was 1-100 mg·L^-1 (r = 0.999 9, n = 6). The average recovery was 100.2 %, and RSD was 2.3 % (n = 9). Conclusions The method is stable, precise and can be applied to the determination of xanthiside.