一株冬虫夏草来源真菌的鉴定及抑菌活性检测

目的:对分离自青海省果洛州冬虫夏草子座内的一株真菌(Cs-ZZ23)进行鉴定,并对其抑菌活性作初步探究。方法:利用形态学和多基因系统分析相结合的方法进行鉴定,同时采用对峙法和改进的菌块法测定了该菌株对6株供试菌的抑制作用。结果:形态特征表明,该菌株与被孢霉属(Mortierella sp.)中的高山被孢霉(Mortierella alpina)的特征一致,多基因序列分析显示本菌株与多个高山被孢霉菌株同源性在99%,因此确定该菌株为高山被孢霉,而且对大肠埃希菌(Escherichia coli),枯草芽孢杆菌(Bacillus subtilis)和瓜果腐霉(Pythium aphanidermatum)有抑制作用。结论:菌株Cs-ZZ23为高山被孢霉,其次级代谢产物具有重要研究价值。

Identification of Fungi from Cordyceps sinensis and Its Antibacterial Activity

Objective: To identify and explore the antimicrobial activity of the fungi (Cs-ZZ23) isolated from the Cordyceps sinensis collected from Guoluo, Qinghai province. Method: The morphology and multilocus phylogenetic analysis were used for identification of the fungi. Meanwhile, 6 strains were used as indicators to test the antimicrobial activity of this fungus using agar disk diffusion method and improved agar block method. Result: The morphological characteristics of this strain were similar to those of Mortierella alpina in M. sp., and the polygenes sequence analysis showed that the sequence homology was 99% between strain Cs-ZZ23 and the multiple strains of M. alpina.Therefore, this strain was determined as M. alpina and it exhibited antibacterial activity against E. coli, Bacillus subtilis and Pythium aphanidermatum. Conclusion: The strain Cs-ZZ23 is a kind of M. alpina and its secondary metabolites deserve to be further developed.