| 大白鼠海马CA_1区锥体细胞的急性分离及其电生理学特性 |
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| 引用本文： | 张宗明,吴才宏,阳冬梅,庄道斌,周培爱,周孝思. 大白鼠海马CA_1区锥体细胞的急性分离及其电生理学特性[J]. 北京大学学报(医学版), 1997, 0(1) |
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| 作者姓名： | 张宗明 吴才宏 阳冬梅 庄道斌 周培爱 周孝思 |
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| 作者单位： | 北京医科大学第三医院外科!100083(张宗明，周孝思)，北京大学生物膜与膜生物工程国家重点实验室(吴才宏，阳冬梅，庄道斌，周培爱) |
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| 摘 要： | 目的：建立适用于膜片组技术（Patchclamptechnigue，PCT）的大白鼠海马CA_4区锥体细胞（rathippocampalCApyramidalneuron，RHCPN）的急性分离方法，并测定该细胞的电生理学特性。方法：用7～21d大鼠，以链霉蛋白酶E酶解加吸管吹打法制备RHCPN，用全细胞PCT测定其电生理学特性。结果：分离的RHCPN易用于PCT，且具有正常的电生理学特性，并保存了主要的电压依赖性和受体门控性高于通道特性。结论：本法分离的RHCPN适用于PCT电生理学研究。
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| 关 键 词： | 海马 锥体细胞／分离和提纯 电生理学 膜片钳 |
ACUTE ISOLATION AND ELECTROPHYSIOLOGICAL PROPERTIES OF CA_1 PYRAMIDAL NEURONS IN HIPPOCAMPUS OF RATS |
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| ZHANG Zongmingh, WU Caihong, YANG Dongmei, ZHUANG Daobin,ZHOU Peiai, ZHOU Xiaosi. ACUTE ISOLATION AND ELECTROPHYSIOLOGICAL PROPERTIES OF CA_1 PYRAMIDAL NEURONS IN HIPPOCAMPUS OF RATS[J]. Journal of Peking University. Health sciences, 1997, 0(1) |
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| Authors: | ZHANG Zongmingh WU Caihong YANG Dongmei ZHUANG Daobin ZHOU Peiai ZHOU Xiaosi |
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| Abstract: | To acutely isolate rat hippocampal CA1 pyramidal neurons (RHCPN) suitable forpatch clamp technique (PCT), and to measure their electrophysiological properties. Methods:RHCPNwere obtained by pronase E treatment and then by trituration with pipette from 7 to 21 day-old rats.PCT was used to study their electrophysiological properties. Results:The isolated RHCPN were easy torecord by PCT. Their electrical and chemical membrane preperties concerning some major voltage -de-pendent and receptor-gated ionic channels were well-preserved. ConcIusion: The RHCPN isolated bythis method is suitable for PCT electrophysiological study. |
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| Keywords: | Hippocampus Pyramidal cells/isol Electrophysiology Patch clamp |
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