E-cad和VEGF及PCNA表达与胆囊癌生物学特性的相关性研究

目的:研究不同胆囊疾病中E钙黏蛋白(E-cad)、血管内皮生长因子(VEGF)及增殖细胞核抗原(PCNA)的表达和分布的差异,探讨E-cad、VEGF和PCNA表达与胆囊癌临床病理特征的相关性及其预后判断的价值。方法:收集胆囊癌标本41例,胆囊腺瘤标本14例,慢性胆囊炎10例,应用免疫组化染色SP法分析。结果:胆囊癌VEGF阳性表达率(33/41)显著高于胆囊腺瘤(6/14)和胆囊炎(0/10),F=24·831,P=0·000,而E-cad阳性表达率(18/41)明显降低,伴有转移者E-cad表达(12/30)更低;胆囊癌PCNA LI高于胆囊腺瘤和胆囊炎组,H=19·03,P=0·008。VEGF过度表达者3年生存率(6/25)较阴性组(5/6)显著降低,F=8·286,P=0·008,PC-NA LI≥50者3年生存率(1/16)较<50者(10/15)显著降低,F=16·151,P=0·000;E-cad高表达者3年生存率(9/16)较阴性组(1/15)明显增高,F=12·511,P=0·001。结论:E-cad和VEGF及PCNA表达在胆囊癌、胆囊腺瘤与慢性胆囊炎之间差异均有统计学意义,均可作为评估胆囊良、恶性肿瘤的参考指标和胆囊癌预后判断的参考指标。

Expressions of E-cad, VEGF and PCNA in gallbladder carcinoma and their correlation with clinical outcome

OBJECTIVE:To study the patterns of the expressions of E-cad, VEGF and PCNA in gallbladder carcinoma,adenoma and chronic cholecystitis, and explore the correlation of E-cad, VEGF and PCNA expressions with gallbladder carcinoma.METHODS:Studies were performed in 41 samples of gallbladder carcinoma, 14 cases of gallbladder adenoma and 10 cases of chronic cholecystitis with immunohistochemistry.RESULTS:The positive rate of VEGF (33/41) in gallbladder carcinoma was significantly higher than rates of gallbladder adenomas and chronic cholecystitis, F=24.831,P=0.000, the PCNA LI in gallbladder carcinoma was significantly hingher than rates of gallblcdder adenomas and chronic cholecystitis, H=19.030, P=0.008. The positive rate of E-cad (18/41)in gallbladder carcinoma was significant lower than rates of gallbladder adenomas and cholecystitis, F=8.286, P=0.008. Three-year survival rate was significantly lower in gallbladder carcinoma with VEGF(6/25) and PCNA (1/16)over expression, F=16.151,P=0.000, and three-year survival rate in the patients with E-cad positive tumors (9/16) was higher than that of those with negative tumors ( 1/15 ), F=12.511,P=0.001.CONSLUSIONS:The expression of E-cad, VEGF and PCNA are significantly different among gallbladder carcinoma, adenoma and chronic cholecystitis. Therefore, benign or malignant gallbladder tumor may be distinguished by detecting the expressions of E-cad, VEGF and PCNA, and they would be valuable for predicting prognosis of gallbladder carcinomas.