固肾培元方对肾阳虚大鼠听力影响的实验研究

目的观察固肾培元方对肾阳虚大鼠听力的影响,为中医肾与耳的关系提供新的实验资料。方法将耳廓反应灵敏的健康SD大鼠随机选取6只用于空白组,其余24只用于肾阳虚造模。采用肌注氢化可的松注射液的方法造成肾阳虚模型,造模14 d后,分为模型组、中药高剂量组、中药中剂量组、中药低剂量组。治疗组造模结束后开始给药,连续给药2周。实验期间观察大鼠的反应、活动情况、进食量、毛色、大小便等。给药结束后,采用MEB-2200肌电图/诱发电位仪检测大鼠的Ⅰ波、Ⅲ波潜伏期、Ⅰ-Ⅲ波峰间潜伏期及听性脑干反应（ABR）。结果固肾培元方可使肾阳虚大鼠ABR阈值降低,Ⅰ波、Ⅲ波潜伏期、Ⅰ-Ⅲ波峰间潜伏期均缩短,与空白组比较,差异无统计学意义（P〉0.05）。结论固肾培元方能够起到改善听力作用。

Experimental Research of the Impacts on the Hearing for the Rats with Kidney Yang Deficiency Treated with the Formula of Consolidating the Kidney and Cultivating the Primary

Objective To observe the impacts on the hearing for the rats with kidney yang deficiency treated with the formula for consolidating the kidney and cultivating the primary so as to provide the updated experimental data on the relationship between the kidney and ear in terms of traditional Chinese medicine.Methods 6 healthy SD rats with sensitive auricular reaction were selected in a blank group and the rest 24 rats were modeled as kidney yang deficiency rats.The muscular injection of hydrocortisone was used to prepare the model of kidney yang deficiency.In 14 days of modeling,the rats were divided into a model group,a high-dose Chinese medicine group,a middle-dose Chinese medicine group and a low-dose Chinese medicine group.In the treatment groups,at the end of modeling,the medication was prescribed and lasted for 2 weeks.In the experiment,the reaction,activity,food intake,fur color,urination and defecation were observed.At the end of medication,MEB-2200 EMG/evoked potential equipment was adopted to detect the latencies of I wave,Ⅱ wave and I-Ⅲ wave crest as well as the auditory brain stem response(ABR).Results The formula for consolidating the kidney and cultivating the primary reduced ABR threshold for the rats with kidney yang deficiency and shortened the latencies of I wave,Ⅱ wave and I-Ⅲ wave crest.The statistical significant differences did not presented as compared with those in the blank group(P>0.05).Conclusion The formula for consolidating the kidney and cultivating the primary can improve the hearing.