洛伐他汀对氧化修饰低密度脂蛋白刺激U937细胞缺氧诱导因子-1α表达的影响

目的：研究氧化修饰低密度脂蛋白（ox—LDL）刺激U937细胞表达缺氧诱导因子-1α（HIF-1α）的变化，并观察洛伐他汀对其的影响。方法：用油红染色的方法检测泡沫细胞的形成，采用实时定量PCR（Real—timePCR）及蛋白印记杂交技术（Westernblotting）检9n,4细胞HIF-1α表达的变化。结果：未加ox—LDL刺激的U937细胞未见泡沫细胞形成；U937细胞加入ox-LDL刺激24h后，全部转化为泡沫细胞。ox—LDL刺激下，U937细胞HIF-1α表达量显著升高，加入洛伐他汀后，HIF-1α的表达量显著降低，随剂量增加，抑制作用显著增强，有明显的剂量依赖关系。结论：ox-LDL氧化损伤导致巨噬细胞HIF—1α高表达，洛伐他汀可抑制U937细胞HIF-1α的表达，抑制泡沫细胞的形成。

Effects of lovastatin on expression of hypoxia inducible factor 1 alpha on U937 cell induced by oxidized low density lipoprotein

Objective：To study the effects of expression of hypoxia inducible 1 alpha （ HIF-1 α） on U937 cell stimulated by oxidized low density lipoprotein（ox-LDL） and the influence of lovastatin. Methods： Foam cell formation was tested by oil red O-stain a- nalysis, and expression of HIF-1α was measured by the method of Real-time PCR and Western blotting. Results： Foam cell was not formed by U937 cell without ox-LDL stimulating; U937 cell with ox-LDL stimulating for 24 h was transformed to foam cell. After treated with ox-LDL, the expression of HIF-1 αwas markedly increased on U937 cells; with the combined treatment of lovastafin ox-LDL, the expression of HIF-1 αwas significantly decreased, and this inhibitory effect was dose dependant. Conclusion：Lovasatin could inhibit both the expression of HIF-1α on U937 and the foam cell formation induced by ox-LDL.