| 化学—酶法立体控制合成光学纯L-羟基苯丙氦酸的新方法 |
| |
| 引用本文： | 赵健身,曹家强,杨顺楷. 化学—酶法立体控制合成光学纯L-羟基苯丙氦酸的新方法[J]. 药学学报, 1995, 30(6): 466-470 |
| |
| 作者姓名： | 赵健身 曹家强 杨顺楷 |
| |
| 作者单位： | 中国科学院成都生物研究所,成都 610041;*四川大学生化专业九四届毕业生 |
| |
| 摘 要： | Optically pure L-3(2-hydroxyphenyl) alanine(L-o-tyrosine ,Ⅲa,),L-3-(3-hydroxyphenyl) alanine(L-m-tyrosine,Ⅲb )and L-3-(4-hydroxyphenyl )alanine(L-p-tyrosine,Ⅲc )were synthesized by the stereocontrolled amination of corresponding hydroxycinnamic acld(Ⅱ)catalyzed by L-phenylalanine ammonia-lyase(PAL,EC4.3.1.5 )contained in Rhodoterula rubramycelium. The amination of compound Ⅱ was completed in aqueous ammonia solution( 6.4mol·L-1,pH10.5, 30℃) with the conversion of 74.9%(Ⅱa),21.1%(Ⅱb)and 20.6%(Ⅱc)respectively.The absolute configuration of the products Ⅲa～c were confirmed by circular dichroism(CD),and chiral high-performance ligand exchange chromatography(HPLEC)showed that productsⅢ were optically pure L-isomers.
|
| 关 键 词： | 化学—酶法合成 苯丙氨酸解氨酶 L-邻-酪氨酸 L-间-酪氨酸 L-对-酪氨酸 |
| 收稿时间： | 1994-08-30 |
A NEW CHEMOENZYMATIC STEREOCONTROLLED SYNTHESIS OF OPTICALLY PURE L-HYDROXYPHENYLALANINE |
| |
| JS Zhao,JQ Cao and SK Yang. A NEW CHEMOENZYMATIC STEREOCONTROLLED SYNTHESIS OF OPTICALLY PURE L-HYDROXYPHENYLALANINE[J]. Acta pharmaceutica Sinica, 1995, 30(6): 466-470 |
| |
| Authors: | JS Zhao JQ Cao SK Yang |
| |
| Abstract: | Optically pure L-3(2-hydroxyphenyl) alanine(L-o-tyrosine ,Ⅲa,),L-3-(3-hydroxyphenyl) alanine(L-m-tyrosine,Ⅲb )and L-3-(4-hydroxyphenyl )alanine(L-p-tyrosine,Ⅲc )were synthesized by the stereocontrolled amination of corresponding hydroxycinnamic acld(Ⅱ)catalyzed by L-phenylalanine ammonia-lyase(PAL,EC4.3.1.5 )contained in Rhodoterula rubramycelium. The amination of compound Ⅱ was completed in aqueous ammonia solution( 6.4mol·L-1,pH10.5, 30℃) with the conversion of 74.9%(Ⅱa),21.1%(Ⅱb)and 20.6%(Ⅱc)respectively.The absolute configuration of the products Ⅲa～c were confirmed by circular dichroism(CD),and chiral high-performance ligand exchange chromatography(HPLEC)showed that productsⅢ were optically pure L-isomers. |
| |
| Keywords: | Chemoenzymatic synthesis L-o-tyrosine L-m-tyrosine L-p-tyrosine L-phenylalanine ammonia-lyase(PAL,EC4.3.1.5) |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《药学学报》浏览原始摘要信息 |
|
点击此处可从《药学学报》下载全文 |
|
