实验动物金黄色葡萄球菌的实验室检测能力验证结果评价

目的 通过实施实验动物金黄色葡萄球菌检出能力验证计划,了解实验动物检测机构检验能力,提高实验动物质量检测水平。方法 按照CNAS批准的能力验证方案,通过低温冻干制备样品,经过稳定性和均匀性检验合格,作为能力验证样品。采用随机编号,发样给参加单位,并附作业指导书。在规定时限提交检验报告和原始记录复印件,其结果与样品预检结果一致的判为满意结果,不一致或未能提交结果的判为不满意结果。结果 共有28个实验室参加本次能力验证项目,其中获得满意验证结果的实验室为22个,占总参加机构的78.57%;未得到满意验证结果的实验室为6个,占21.43%。采用国标检测方法的有27个,采用PCR检测方法的有1个。结论 实验动物质量检测机构对金黄色葡萄球菌的检测能力较高,实施能力验证计划能够反映实验室的检测水平。

Evaluation of the ability to detect Staphylococcus aureus in experimental animal laboratories

Objective To verify the detection ability of experimental animal quality detection laboratories in China for Staphylococcus aureus. Methods The testing samples for Staphylococcus aureusdetection were prepared by bacterial culture, homogeneity test and stability test, according to the study plan approved by CNAS. Then the samples and operation instruction were sent to the participant laboratories. The detection reports from these laboratories should be submitted before the deadline expires, and the collected data were summarized and analyzed. Results There were 28 laboratories which joined to this test plan. Among them 22 laboratories(78.57%) achieved satisfactory test results, and six laboratories(21.43%) had unsatisfactory test results. 27 Laboratories used the national standard detection assay, while only one laboratory used PCR assay. Conclusions Most of experimental animal quality testing laboratories in China have sufficient proficiency in detection of Staphylococcus aureus. The obtained information are very helpful for the laboratory ability verification testing in future.