| KCL22/NOD-SCID小鼠慢性粒细胞白血病移植瘤模型的建立及其鉴定 |
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| 引用本文： | 文良雪,刘鑫,李会,黄宁姝,黄峥兰,冯文莉. KCL22/NOD-SCID小鼠慢性粒细胞白血病移植瘤模型的建立及其鉴定[J]. 实验动物与比较医学, 2015, 23(2): 188-193 |
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| 作者姓名： | 文良雪 刘鑫 李会 黄宁姝 黄峥兰 冯文莉 |
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| 作者单位： | 重庆医科大学检验医学院,重庆医科大学检验医学院,重庆医科大学检验医学院,重庆医科大学检验医学院,重庆医科大学检验医学院,重庆医科大学检验医学院 |
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| 摘 要： | 目的：研究人慢性粒细胞白血病细胞株KCL22在NOD-SCID小鼠体内致白血病的能力，为慢性粒细胞白血病血液移植瘤模型鼠的建立奠定基础。方法：取对数生长期的KCL22细胞2×107个，经尾静脉注射入NOD-SCID小鼠，对照组小鼠注射无菌PBS。观察小鼠一般情况，瑞氏染色监测血象和骨髓象变化，PCR检测骨髓细胞BCR-ABL基因转录水平，HE染色观察肝、脾组织肿瘤细胞浸润情况。结果：实验组小鼠于注射细胞后约4周开始出现反应力下降、精神萎靡、股骨肌肿大、后肢骨节出血点等体征，外周血白细胞从第5周逐渐增多，计数较对照组显著升高（P＜0.05），血涂片可见幼稚粒细胞，肝、脾、骨髓组织切片可见白血病细胞浸润，骨髓细胞高表达BCR-ABL融合基因，未经治疗存活约70天，较对照组显著缩短（P＜0.05）。结论：KCL22细胞可成功构建NOD-SCID小鼠慢性粒细胞白血病移植瘤模型。
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| 关 键 词： | 慢性粒细胞白血病；KCL22细胞；NOD-SCID小鼠；白血病模型鼠 |
| 收稿时间： | 2014-10-30 |
| 修稿时间： | 2014-11-19 |
Establishment and verification of a KCL22/NOD-SCID mouse transplantation tumor model of chronic myeloid leukemia |
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| WEN Liang-xue,LIU Xin,LI Hui,HUANG Ning-shu,HUANG Zheng-lan and FENG Wen-li. Establishment and verification of a KCL22/NOD-SCID mouse transplantation tumor model of chronic myeloid leukemia[J]. Laboratory Animal and Comparative Medicine, 2015, 23(2): 188-193 |
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| Authors: | WEN Liang-xue LIU Xin LI Hui HUANG Ning-shu HUANG Zheng-lan FENG Wen-li |
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| Affiliation: | College of Laboratory Medicine, Key Laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China;College of Laboratory Medicine, Key Laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China;College of Laboratory Medicine, Key Laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China;College of Laboratory Medicine, Key Laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China;College of Laboratory Medicine, Key Laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China;College of Laboratory Medicine, Key Laboratory of Medical Diagnostics, Ministry of Education, Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | |
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| Keywords: | Chronic Myeloid Leukemia (CML) KCL22 cells NOD-SCID mice Leukemia animal model |
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