两品系大鼠在经典条件反射和操作式条件反射中的行为学表现

目的 探讨SD、Wistar两种品系大鼠在经典条件反射和操作式条件反射中行为学表现差异,为研究脑的高级功能建立稳定的条件反射动物模型提供实验动物选择依据.方法 选择8周龄的正常SD和Wistar大鼠,分别进行奖赏训练、单次操作训练、连续多次操作训练三种检测模式,研究SD、Wistar大鼠学习记忆能力.结果 奖赏训练中,SD大鼠的鼻触次数显著性增多(vs.Wistar,P<0.05);单次操作训练中,与SD大鼠比较,Wistar大鼠的总踏板次数(LPs)、比率(cLP/LP)显著升高(P<0.05),总鼻触次数(NPs)于第5天差异有显著性,踏板潜伏期明显降低(P<0.05),踏板速率则显著性的升高(P<0.05).连续多次操作训练中,Wistar大鼠鼻触次数显著性升高(P<0.05),踏板次数、奖赏次数均增多,反应准确率也高于SD大鼠,但组间差异无显著性(P>0.05).结论 经典条件反射阶段SD大鼠对饮水盒的探索能力强,表现出对奖赏物质较强的兴趣;而在奖赏操作条件反射阶段Wistar大鼠的表现优于SD大鼠.

Behavioral differences of the Sprague-Dawley and Wistar rats in Pavlovian conditioning and reward operant conditioning

Objective To explore the behavioral differences of Sprague-Dawley (SD) and Wistar rats in Pavlovian conditioning and reward operant conditioning task, and provide a good reference for selection of experimental animals in establishing the animal models of conditional reflex for studying higher brain functions. Methods Healthy 8-week-old SD and Wistar rats were used in this study. Single operant task and continuous operation training task, and multiple operation training task were used to assess the learning and memory ability of the SD and Wistar rats. Results Significant difference was found in the Pavlovian conditioning. SD rats performed more nose pokes (NPs) (P<0.05) vs. Wistar rats. There were significant differences during a single operation task.Wistar rats preformed significantly more total lever presses (LPs) (P<0.05) and cLP/LP (P<0.05) vs. SD rats, and there was obvious increase in total nose pokes (NPs) (P<0.05) on the fifth day. The two indicators,the lever pressing rate and the latency of the first lever pressing could explain objectively the rat learning and memory abilities. The latency of the first lever pressing in the Wistar rats was significantly shorter than that of SD rats (P<0.05), while the lever pressing rate obviously higher (P<0.05). In the continuous operation training task, the Wistar rats showed significantly higher nose pokes (vs. SD rats, P<0.05); and also had higher lever presses, reward earned, and correct accuracy rate, but with no significant difference between them.Conclusions Wistar rats exhibit excellently in a single operation task and continuous operation training task, while SD rats show better performance in Pavlovian conditioning.