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Identification of human parvovirus B19 among measles and rubella suspected patients from Cuba |
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| Authors: | María de los Ángeles Ribas Yahisel Tejero Yanislet Cordero Daileny Pérez Aurélie Sausy Claude P. Muller Judith M. Hübschen |
| Affiliation: | 1. Virology Department, National Reference Laboratory of Measles, Mumps, Rubella, Pedro Kourí Institute of Tropical Medicine, Havana, Cuba;2. Department of Infection and Immunity, Infectious Diseases Research Unit, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg;3. Department of Infection and Immunity, Infectious Diseases Research Unit, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg Institute of Immunology, Laboratoire National de Santé, Dudelange, Luxembourg |
| Abstract: | Between September 2014 and December 2015, 298 sera from rash and fever patients from all over Cuba were investigated for specific IgM antibodies against measles, rubella, dengue, human parvovirus B19 (B19V) and human herpesvirus 6 (HHV6) using a commercial enzyme-linked immunosorbent assay kits. B19V IgM positive and equivocal samples were investigated by a polymerase chain reaction and genotyping. No measles, rubella or dengue cases were detected. HHV6-IgM antibodies were confirmed in 5.7% and B19V-IgM antibodies in 10.7% of the patients. A total of 31.3% of the B19V cases were between 5 and 9 years old and 34.4% were 20 years and older. The only B19V sequence obtained belonged to genotype 1a. Diagnosis was established for only 16% of the rash and fever patients, suggesting that other diseases such as Zika or Chikungunya may play a role. |
| Keywords: | Cuba human parvovirus B19 rash-fever |