条件性感染金黄色葡萄球菌关节炎小鼠模型的建立和分析

目的 建立与分析条件性感染金黄色葡萄球菌感染关节炎模型，为关节炎机理研究及药物开发提供动物模型。方法 先对小鼠进行环磷酰胺免疫抑制处理，再尾静脉接种金黄色葡萄球菌，通过关节表观特征观察、关节炎指数分析和病理观察评分对模型进行评价。结果 接种后第1天开始，小鼠关节部位轻微肿胀，关节组织病变由少量滑膜增生且排列疏散紊乱，发展至滑膜增厚，往关节腔内延伸生长，中性粒细胞浸润增多等病理变化。结论 成功建立条件性感染SA模型，能在较短时间内模拟人关节炎的临床症状和病程发展，且在感染和免疫机理方面体现了人关节炎发病病因。

Establishment and analysis of a mouse model of Staphylococcus aureus-induced arthritis

Objective To establish and analyze a mouse model of Staphylococcus aureus-induced arthritis (Staphylococcus aureus septic arthritis, SA), and provide an animal model for arthritis mechanism research and drug development. Methods Mice were immunosuppressed with cyclophosphamide, then intravenously inoculated with Staphylococcus aureus. The gross characteristics of the joints were observed, the arthritis indexes were analyzed, and the pathological scores of the model mice were evaluated. Results From the first day after bacterial inoculation, the mouse joints were swollen. Pathological examination revealed lesions varying from mild and disarranged joint synovial hyperplasia to synovial thickening and intra-articular invasion, and increased neutrophil infiltration. Conclusions A mouse model of Staphylococcus aureus-induced arthritis is successfully established in this study. This model can be developed in a relatively short time, can not only simulate the clinical symptoms and signs and disease progression of human arthritis, but also to a certain extent reflects the etiology, infection and immunological mechanisms of human arthritis.