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| 鼠输尿管完全梗阻后肾脏病理及足细胞表型改变的研究 | |
| 引用本文： | 于雪馨,王常林,孙荣国,杨屹. 鼠输尿管完全梗阻后肾脏病理及足细胞表型改变的研究[J]. 中国医科大学学报, 2010, 39(1): 1-3 |
| 作者姓名： | 于雪馨 王常林 孙荣国 杨屹 |
| 作者单位： | 1. 中国医科大学,附属盛京医院,小儿内科,沈阳,110004 2. 中国医科大学,附属盛京医院,小儿泌尿外科,沈阳,110004 |
| 基金项目： | 国家自然科学基金资助项目(30672192) |
| 摘 要： | 目的观察鼠输尿管完全梗阻后肾脏病理和足细胞表型改变的时序变化,寻找输尿管梗阻后肾脏发生不可逆损伤的时相。方法建立单侧输尿管完全梗阻动物模型,梗阻后不同时间点取出肾脏,经HE染色和免疫荧光双标方法,观察肾脏病理及足细胞表型的改变。结果大体观察,梗阻24h后出现肾盂扩张,梗阻3d后出现肾皮质变薄。组织学观察,梗阻12h后出现一部分近曲小管扩张,小管上皮细胞呈水样变性肿胀,在梗阻3d后出现大量小管上皮细胞变形肿胀。免疫荧光双标法,梗阻24h后有少量足细胞、巨噬细胞同时被2种抗体标记,2d后足细胞呈减少趋势,而巨噬细胞逐渐增多。结论输尿管梗阻后在肾脏病理改变早于肾脏发生形态学改变的发生,输尿管梗阻后24h发生足细胞表型的改变,与肾脏肾盂出现明显扩张的时间相近,随着梗阻时间的延长,肾脏病理改变逐渐加重,足细胞转化逐渐增多。 |
| 关 键 词： | 输尿管梗阻 足细胞表型 肾脏损伤 |
Renal Pathological Appearance and Podocyte Phenotype after the Ureteral Obstruction |
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| YU Xue-xin , WANG Chang-lin , SUN Rong-guo , YANG Yi. Renal Pathological Appearance and Podocyte Phenotype after the Ureteral Obstruction[J]. Journal of China Medical University, 2010, 39(1): 1-3 | |
| Authors: | YU Xue-xin WANG Chang-lin SUN Rong-guo YANG Yi |
| Affiliation: | YU Xue-xin1,WANG Chang-lin2,SUN Rong-guo2,YANG Yi2 (Department of Pediatrics,2. Department of Pediatric Surgery,Shengjing Hospital,China Medical University,Shenyang 110004,China) |
| Abstract: | Objective To find time point of the irreversible kidney injury by observing renal pathological appearance and podocytes phenotype after complete ureteral obstruction. Methods The animal model of complete unilateral ureteral obstruction was constructed in 2-weekold and 2-month-old rats respectively. HE staining was employed to evaluate the degree of pathological changes,and immunofluorescence double staining to observe the transitional progression of podocyte. Results Grossly,there appeared pyelectasis at 2 ... |
| Keywords: | unilateral ureteral obstruction podocyte cell transition renal injury |
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