| 甘氨脱氧胆酸盐诱导人正常肝细胞株HL-7702凋亡过程中caspase-3，8，9蛋白活性变化及凋亡机制研究 |
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| 引用本文： | 传良敏,杨永长,黄文芳,饶绍琴,洪华,邓君.甘氨脱氧胆酸盐诱导人正常肝细胞株HL-7702凋亡过程中caspase-3，8，9蛋白活性变化及凋亡机制研究[J].四川医学,2010,31(1):10-12. |
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| 作者姓名： | 传良敏 杨永长 黄文芳 饶绍琴 洪华 邓君 |
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| 作者单位： | 四川省人民医院检验科,四川,成都,610072 |
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| 摘 要： | 目的观察甘氨脱氧胆酸盐（glycodeoxycholate，GCDC）诱导人正常肝细胞株HL．7702凋亡过程中caspase．3，8，9活性的变化，探讨其凋亡机制。方法体外培养HL-7702细胞，以GCDC为处理因素，用AnnexinV．F1TC／PI双染色法结合流式细胞技术仪检测细胞凋亡率，比色法测定caspase-3，8，9活性。结果100、150、200、250μmol／L的GCDC处理HL-7702细胞24h后以及250μmol／LGcDc处理细胞4、8、12、24h后，其细胞凋亡率、caspase-3，8，9活性明显升高，与对照组比较，差异有统计学意义（P〈O．05）。结论GCDC可通过活化caspase-8，9，进而激活caspase-3从而诱导肝细胞凋亡。
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| 关 键 词： | 甘氨脱氧胆酸盐 细胞凋亡 caspase |
Changes in caspase-3,8,9 activities of normal human hepatocyte HL-7702 cell in the process of apoptosis induced by glycodeoxycholate |
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| Institution: | CHUAN Liang-min, YANG Yong-chang, HUANG Wen-fang, et al. ( The People＇s Hospital of Sichuan, Chengdu , Sichuan 610072, China) |
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| Abstract: | Objective To investigate the apoptosis and changes of caspase-3,8,9 activities in cultured normal human hepatocyte HL-7702 cells induced by glycodeoxycholate(GCDC)in vitro and to explore the possible mechanism of hepatocyte apoptosis.Methods HL-7702 cells were cultured with GCDC.The apoptosis ratio of HL-7702 cells was determined by Annexin V-FITC/PI double staining.Colorimetric method was used to measure caspase-3,8,9 activities.Results When hepatocytes were incubated with GCDC for 24h,the apoptotic ratio ... |
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| Keywords: | caspase |
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