乙型肝炎病毒在原发性肝细胞癌中病原意义的研究

本文收集我院近年经病理确诊的肝细胞癌70例、毛细胆管型肝癌7例,复核临床材料和HE染色切片,同时再作Victoria blue染色观察肝细胞内HBsAg,证明未查血清HBsAg、血清HBsAg阴性和阳性者肝细胞内HBsAg检出率分别为85.7％、75.0％和90.2％,三者无明显差别,仅血清HBsAg阴性者肝细胞内HBsAg数量明显少些,提示肝细胞癌病例呈血清内HBsAg阴性,并不能除外HBV感染和致癌可能。再者,34.3％标本内可在肝癌细胞内查见HBsAg,9.8％标本于癌、肝组织间不正常肝细胞内查见HBsAg,而7例毛细胆管型肝癌,HE染色肝组织内未见毛玻璃样肝细胞,Victoria blue染色未见HBsAg,进一步说明HBV感染与肝细胞癌问确具密切因果关系。

STUDY OF THE ETIOLOGICAL SIGNIFICANCE OF HBV IN PRIMARY HEPATOCELLULAR CARCINOMA

The clinical data and HE stain sections of 70 PHC cases and 7 bile-capillary carcinoma patients were reviewed. Victoria blue stain was used for all these liver samples,. Our observations showed that the detection rates of HBsAg in liver cells without a history of positive serum HBsAg test and either with serum HBsAg negative or positive were rather high, 85.7%. 75.0% and 90.2% respectively. There was no significant difference among them. It is worthy to note that HBsAg could be detected in liver cells sections with Victoria blue stain in 75.0% of liver samples whose serum HBsAg was negative. The positive rates of HBsAg in liver cells of serum HBsAg negative and positive patients were without obvious difference. It seemed to suggest that the posibility of HBV infection and its carcinogenic role could not be excluded in the serum HBsAg negative patient. In addition, HBsAg could be detected in the carcinoma cells in 34.3% of liver samples, and also in the abnormal cells between the carcinoma and liver tissue sections in 9.8% with Victoria blue stain. However, neither HBsAg could be found in all the 7 bile-capillary carcinoma sections with in Victoria blue stain, norground-glass cells could be seen in the HE stain sections. These results seemed to further prove that there might be a close cause and effect relationship between the HBV infection and PHC.